Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is organic
Patent
1989-01-13
1991-11-26
Rosen, Sam
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is organic
435 71, 435 72, 435 75, 436519, 436520, 436522, 436529, 436531, 436829, G01N 33549
Patent
active
050681982
ABSTRACT:
Assay methods and compositions are provided for determining an analyte in a sample suspected of containing the analyte. The composition comprises in a novel single liquid reagent at least one specific binding pair (sbp) member and its complementary member wherein at least one sbp member is reversibly confined in a material that temporarily renders the confined sbp member incapable of binding with its complementary sbp member. At least one of the sbp members is bound to a member of a signal producing system capable of producing a detectable signal in relation to the amount of analyte in the sample. The confinement is reversed, any remaining members of the signal producing system are added, and the signal produced in relation to the amount of analyte is measured. Examples of the confining material are lipid bilayers, cells and gels.
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Felgner Philip L.
Gibbons Ian
Ullman Edwin F.
Barrett Carol F.
Precivale Shelley G.
Rosen Sam
Swiss Gerald F.
Syntex (U.S.A.) Inc.
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