Liquid single reagent for air enzyme complementation assay

Details Liquid single reagent for air enzyme complementation assay Liquid single reagent for air enzyme complementation assay

1988-06-22

1991-07-16

Saunders, David A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 78, 435 18, 435961, 435962, 435963, 435975, 436500, 436501, 436505, 436537, 436826, G01N 33535, G01N 33542, G01N 33566

Patent

active

050325035

ABSTRACT:
A method is provided for combining normally interacting reagents in a liquid single reagent and preventing complex formation using a surfactant and then reversing the inhibition by adding a cyclodextrin. The method finds particular use in diagnostic immunoassays. Reagents facilitating the invention are also provided.

REFERENCES:
patent: 4121975 (1978-10-01), Ullman et al.
patent: 4447527 (1984-05-01), Monte et al.
patent: 4708929 (1987-11-01), Henderson
patent: 4786591 (1988-11-01), Draeger et al.
A. Hersey et al., J. Chem. Soc., Faraday Trans 1, 1986, 82: 1271-1287, Mechanism of Inclusion-Compound Formation for Binding of Organic Dyes, Ions, and Surfactants to .alpha.-Cyclodextrin by Kinetic Methods & Based on Competition Ex. Short Communication, Effects of Detergents on the Hydrolysis of Glycolipids by .beta.-Galactosidase.
Daniel R. Henderson et al., Clinical Chemistry (1986) 32: 1637-1641, CEDIA.TM., a New Homogeneous Immunoassay System.
Dimitriadix, Giorgios J., "Effect of Detergents on Antibody-Antigen Interaction", in Analytical Biochemistry (1979) 98: 445-451.
Chemical Abstracts, vol. 106, No. 3, Jun. 19, 1987, p. 645, Ab. 106: 18963h, Hersey et al., "Mechanism of Inclusion . . . ".
Chemical Abstracts, vol. 104, No. 15, Apr. 14, 1986, Ab. 104: 125872h, Bachas et al., "Binding Proteins as . . . ".

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