Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Stablizing an enzyme by forming a mixture – an adduct or a...
Patent
1982-12-09
1985-02-05
Shapiro, Lionel M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Stablizing an enzyme by forming a mixture, an adduct or a...
25217412, C12N 996, C11D 742
Patent
active
044978979
ABSTRACT:
A storage stable liquid enzyme concentrate of Subtilisin Carlsberg containing 0.5-6.5 Anson Units of proteinase per gram of concentrate and method for preparing same. Solid form proteinase is extracted with 70-100 parts by volume propylene glycol, 30-0 parts by volume of water, then adjusted as necessary to 60-85% by wt. of the glycol. Stabilizing agents in the concentrate are 0.1-1 mol/kg. of one or more C.sub.1 -C.sub.3 carboxylate, formate being preferred, and a calcium ion content of 0.04-0.5% by wt., pH range is 5-8.
REFERENCES:
patent: 3717550 (1973-02-01), Ziffer
patent: 4111855 (1978-09-01), Barrat et al.
patent: 4305837 (1981-12-01), Kaminsky et al.
patent: 4318818 (1982-03-01), Letton et al.
Aunstrup, K., Proteinases in Economic Microbiology, vol. 5, Microbial Enzymes and Conversions, A. H. Rose (Ed.), pp. 55-65 (1980).
Eilertsen Jens H.
Fog Arne D.
Gibson Keith
Huleatt Jayme A.
Novo Industri A/S
Shapiro Lionel M.
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