Liquid proteinase concentrate and method for preparation

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Stablizing an enzyme by forming a mixture – an adduct or a...

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252132, 252153, 25217412, 252544, 252546, 252DIG12, 435222, C11D 320, C11D 332, C11D 3386, C12N 954

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active

045433330

ABSTRACT:
A storage stable liquid enzyme concentrate of Subtilisin Carlsberg containing 0.5-6.5 Anson Units of proteinase per gram of concentrate and method for preparing same. Solid form proteinase is extracted with 70-100 parts by volume propylene glycol, 30-0 parts by volume of water, then adjusted as necessary to 60-85% by wt. of the glycol. Stabilizing agents in the concentrate are 0.1-1 mol/Kg of a member selected from the group consisting of Na, K, and Ca glutamates, and glycinates, and acetamide, and also a calcium ion content of 0.04-0.5% by wt., pH range is 5-8.

REFERENCES:
patent: 3050445 (1962-08-01), Damaskus
patent: 3051627 (1962-08-01), Bradford
patent: 3296094 (1967-01-01), Cayle
patent: 3325364 (1967-06-01), Merritt
patent: 3560392 (1971-02-01), Eymery
patent: 3707505 (1972-12-01), Maeda
patent: 4305837 (1981-12-01), Kaminsky
patent: 4318818 (1982-03-01), Letton
Research Disclosure, No. 277, May 1982, p. 170, #21751.

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