Chemical apparatus and process disinfecting – deodorizing – preser – Control element responsive to a sensed operating condition
Patent
1996-11-20
1999-04-20
Scheiner, Toni R.
Chemical apparatus and process disinfecting, deodorizing, preser
Control element responsive to a sensed operating condition
422100, 436526, 4352864, 4352865, 4352877, 4352878, 4353091, C12M 132, G01N 3353, B01L 302
Patent
active
058956310
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
The present invention relates to a liquid processing method making use of a pipette device and an apparatus for the same, with which works including for quantifying, separating, taking out, pipetting, clarifying, condensing, diluting a liquid or a target high molecular substances included in a liquid such as useful substances such as antibiotic substance, genetic substances such as DNA, and immunological substances such as antibodies, and/or works for extracting, recovering and isolating the target high molecular substance can automatically and accurately be executed by means of absorbing and discharging the liquid through a liquid absorbing/discharging line in the pipette device.
BACKGROUND ART
In recent years, research activities for DNA or the like are very active in many fields including engineering, medical science, agriculture, physical science, pharmacology, and the purpose includes genome sequencing, clinical diagnosis, improvement of agricultural products, bacteriological inspection of foods, drug preparing systems or the like.
As described above, when various types of immunoassay applicable in a very wide range with high expected possibility in its application or structural analysis of molecular level organisms, microorganisms, or substances such as cells, DNA, RNA, mRNA, plasmid, virus, or bacteria (described simply as a target high molecular substance in the present specification) is performed, it is necessary to carry out with high precision works such as those for quantifying, separating, taking out, pipetting, clarifying, condensing, diluting a sample or a target high molecular substance included in the sample or works for extracting, recovering, or isolating a target high-molecular substance as a preprocessing.
To explain structural analysis of a gene such as DNA diagnosis as an example, at first it is necessary to extract, recover, and isolate a DNA region including a target gene. The technology for extracting, recovering, and isolating genes has already been established as the gene cloning technology or genome sequencing technology, and at present it is believed that, by spending enough time and expenses, any gene can be separated and obtained. For this reason, if a target gene DNA has been extracted, recovered, and separated, any type of gene analysis is possible as a principle by making use of the separated gene DNA.
However, in a case of man, for instance, a particular target gene DNA is one millionth or below of all genome DNA, and for this reason actually a quantity of DNA obtainable for testing is quite small, while a quantity of DNA and RNA not necessary for a particular experiment is quite large, which makes it difficult to execute analysis smoothly.
For the reasons as described above, to execute structural analysis of a gene such as DNA diagnosis, it is important to extract, recover, and isolate a DNA area including a target gene. Description is made hereinafter for a basic method of extracting, recovering, and isolating a DNA.
A DNA exist in a nucleus as a complex with a protein in a cell. In the basic sequence for extracting a DNA, a cell or a cell nucleus is processed with SDS (surfactant dodecil sodium sulfate) to make the DNA soluble, and proteins contained therein are removed with a proteolytic enzyme or phenol.
In other words, when a DNA is separated from the tissue, at first the tissue taken out is put in ice and kept therein for a certain period of time under a low temperature, then this cooled tissue is divided to small pieces each with the weight of around 0.1 g, which are washed with a ice-cooled buffer solution A (0.01M Tris HCl, pH 7.8, 0.1M NaCl, 2 mM MgCl.sub.2). This tissue is put in the above-described buffer solution A having a volume 20 times larger as compared to that of the tissue and is homogenized 5 or 10 times with a Potter type homogenizer. Then the tissue is put in a centrifugal tube together with the buffer solution and is subjected to centrifugation (2,000 rpm, for 5 minutes). The cell nucleus or the cell precipitates, so that the
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Precision System Science Co., Ltd.
Scheiner Toni R.
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