Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-11-28
1997-06-10
Housel, James C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 771, 435 28, 435188, 436536, G01N 33536
Patent
active
056374739
ABSTRACT:
Reagents for a liquid-phase immunodiagnostic assay (LIDA) method comprise a first enzyme; a second enzyme; a first agent which is capable of binding with an analyte to form a complex, the agent being attached to one of the first and second enzymes; and a complex-binding agent attached to the remaining enzyme, wherein the first enzyme is capable of interacting with a substrate for the first enzyme together with any necessary additional substrates for the first enzyme to produce a substrate for the second enzyme, and wherein the second enzyme is capable of interacting with the substrate produced by the first enzyme together with any necessary additional substrates, such that occurrence of the second of the interactions is detectable. The reagent optionally further comprises a scavenger substance capable of inactivating the substrate produced by the first enzyme.
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Housel James C.
King Theresa
University of Florida Research Foundation Inc.
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