Liquid junction coupling for capillary zone electrophoresis/ion

Details Liquid junction coupling for capillary zone electrophoresis/ion Liquid junction coupling for capillary zone electrophoresis/ion

1989-02-16

1991-02-19

Niebling, John F.

Chemistry: electrical and wave energy

Production of desired compound by wave energy in presence of...

Oxygen treating agent

2641801, G01N 2726, B01D 5702

Patent

active

049941658

ABSTRACT:
An improved capillary zone electrophoresis (CZE)/ mass spectrometer interface comprises a liquid junction adapted to makeup the volume/flow rate of the CZE column eluent stream to be more compatible with the ion spray LC/MS interface flow rates required by mass spectrometer detectors in on-line separation/detection systems for variouscompositions and mixtures including biological fluids and environmental samples. Analytes eluting from the CZE capillary column are diluted with a suitable buffer thereby adjusting the volume and flow rates to prevent flow and pressure discrepanies which would otherwise result in peak broadening.
The new liquid junction system for CZE/MS and CZE/MS/MS is useful in separating, detecting and analyzing compositions and mixtures such as amino acids, pesticides, peptides, mucleotides, sulfonated azo dyes and enzymatic digests such as tryptic digest of recombinant bovine somatotropan. Detection can be carried out in both positive and negative ion modes. High separation efficiencies ranging from 50,000 to 1,000,000 theoretical plates and peak asymmetries of 1.2 to 1.8 for tryptic digest are achieved.

REFERENCES:
patent: 3997298 (1976-12-01), McLafferty et al.
patent: 4298795 (1981-11-01), Takeuchi et al.
patent: 4391778 (1983-07-01), Andresen et al.
patent: 4607163 (1986-08-01), Mizuno
patent: 4705616 (1987-11-01), Andresen et al.
patent: 4708782 (1987-11-01), Andresen et al.
patent: 4842701 (1989-06-01), Smith et al.
patent: 4885076 (1989-12-01), Smith et al.
Smith et al, "Improved Electrospray Ionization Interface for Capillary Zone Electrophoresis-Mass Spectrometry", Analytical Chemistry, 1988, 60, 1948-1952.
Udseth et al, "Capillary Isotachophoresis /Mass Spectrometry", Analytic Chemistry, 1989, 226-232.
Lee et al, Biomedical and Environmental Mass Spectrometry, vol. 18, 844-850 (1989).
Kennedy, R.T. et al, Science, 246:57-63 (1989).
Fenn, J.B. et al, Science, 246:64-71 (1989).
Lee, E. et al, J. Chromatogr., 458:313 (1988).
Covey, T.R. et al, Rapid Commun. Mass Spectrom., 2:249 (1988).
Lee, E.D. et al, J. Microcolumn Seperations, 1:14-18 (1989).
Brunis et al., Anal. Chem., 59:2642-2646 (1987).
Olivares, J.A. et al, Anal. Chem., 59:1230-1232 (1987).
Smith, R.D. et al., Anal. Chem., 60:436-441 (1988).
Wallingford, R.A. et al, Anal. Chem., 59:1762-1766 (1987).
Skekhar Hattangadi, Industrial Chemist, pp. 11-12, Feb. 1988.
Smith, R.D. et al, Anal. Chem., 60: 1948-1952, Sep. 15, 1988.
Minard, R.D. et al, paper ROC 945 Capillary Zone Electrophoresis/Continuous Flow FABMS-abstract of presentation 36th Annual Conference on Mass Spectrometry and Allied Topics, Jun. 5-10, 1988, San Francisco, CA.
Lee et al, "On-Line Capillary Zone Electrophoresis/Ion Spray Tandem Mass Spectrometry Determination on Small Peptides"; 36th Conference on Mass Spectrometry and Allied Topic Jun. 5-10, 1988, San Francisco, CA.
Journal of Chromatography, 458:313-321 (1988).
Covey et al, "Capillary Zone Electrophoresis/Mass Spectrometry of Sulfonated Azo Dyes"-36th ASMS Conference on Mass Spectrometry and Allied Topics, Jun. 5-10, 1988, San Francisco, CA.
Brunis et al., "Electrospray LC/MS with an Atmospheric Pressure Ionization Tandem Quadrupole Mass Spectrometer".
Udseth, Harold R. et al., Anal. Chem. 61:228-232 (1989).

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