Liquid detergent with stabilized enzyme

Details Liquid detergent with stabilized enzyme Liquid detergent with stabilized enzyme

1990-07-23

1991-08-13

Lieberman, Paul

Compositions

Water-softening or purifying or scale-inhibiting agents

252DIG12, 252DIG4, 435188, C11D 100, C11D 960, C12N 996

Patent

active

050394460

ABSTRACT:
Liquid detergent containing enzymes particularly proteases are stabilized against enzyme degradation prior to use by inclusion of an inhibitor of the enzyme having a dissociation constant of between about 1.times.10.sup.-2 M and about 1.times.10.sup.-7 M which binds to the enzyme such that prior to use of the detergent at least about 55% of the enzyme is bound to the enzyme inhibitor essentially at the enzyme active site. Such enzyme inhibitor so selected can be used effectively in concentrations much lower than previously taught; i.e., from about 0.002% to less than 0.1% weight/weight.

REFERENCES:
patent: 4566985 (1986-01-01), Bruno
Lindquist, R. N. and Terry, C., "Inhibition of Subtilisin by Boronic Acids, Potential Analogs of Tetrahedral Reaction Intermediates", Archives of Biochemistry and Biophysics, vol. 160 (1974), pp. 135-144, QP501A 77.
C. Abstracts I:85:1676s, Cold Spring Harbor Conf. Cell Proliferation 1975, 2 (Proteases Biol. Control), 429-54.
C. Abstracts II:74:94688h, Proc. Nat. Acad. Sci. U.S., 1971, 68(2), 478-80.

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