Liquid crystalline substrates for culturing cells

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Solid support and method of culturing cells on said solid...

Reexamination Certificate

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C435S007200, C435S325000, C435S408000, C435S007210, C436S501000

Reexamination Certificate

active

07947500

ABSTRACT:
The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e.g., reorganization) of extracellular matrix components by living cells.

REFERENCES:
patent: 2005/010160 (2005-02-01), None
Brake et al., “Biomolecular interactions at phospholipid-decorated surfaces of liquid crystals” 2003 Science 302:2094-2097.
Geiger et al., “Transmembrane crosstalk between the extracellular matrix—cytoskeleton crosstalk” 2001 Nature Reviews 2:793-805.
Balaban et al., “Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates” 2001 Nature Cell Biology 3:466-472.
International Search Report mailed Oct. 15, 2007 for PCT/US2006/061414; Wisconsin Alumni Research Foundation.
Yan-Yeung Luk et al., “Non-Toxic Thermotropic Liquid Crystals for Use With Mammalian Cells” 2004 Liquid Crystals Taylor and Francis, Abingdon, GB vol. 31 No. 5 pp. 611-621.
Li-Lin Cheng et al., “Compatibility of Lyotropic Liquid Crystals with Viruses and Mammalian Cells that Support the Replication of Viruses” 2005 Biomaterials p. 7173-7182.
Besseau et al., “Production of Ordered Collagen Matrices for Three-Dimensional Cell Culture” Biomaterials vol. 23 No. 1 pp. 27-36, 2007.

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