Liquid purification or separation – With means to add treating material – Chromatography
Patent
1995-04-17
1996-08-13
Kim, John
Liquid purification or separation
With means to add treating material
Chromatography
2105021, 210656, 435176, 436527, 502401, 502403, 502405, 530811, B01D 158
Patent
active
055453171
ABSTRACT:
Packing materials for liquid chromatographic or catalytic columns are prepared by contacting a porous protein-adsorptive particulate or membranous support, such as a porous silica particulate support, with an aqueous solution into which a protein has been dissolved to form a saturated coating of protein on the external surfaces of the porous protein-adsorptive support, removing excess protein that remains in solution by washing, and, then crosslinking the protein in the coating. The result is a packing material which resists further adsorption by many different proteins but which continues to provide the adsorptive or catalytic properties of the groups on the internal surfaces of the porous protein-adsorptive support for separations, analysis, or alteration of small molecules. The packing material of the present invention is particularly useful in HPLC or solid phase extraction columns for direct injection drug analysis in plasma, serum, and urine.
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Dow Corning Corporation
Kim John
McKellar Robert L.
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