Liquid chromatography using microporous hollow fibers

Liquid purification or separation – Processes – Liquid/liquid solvent or colloidal extraction or diffusing...

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210656, 2101982, 2105021, 21050023, 55 67, 55386, B01D 1508

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049576208

ABSTRACT:
Liquid chromatography separations of solutes are achieved using porous hollow fibers. The pores of the hollow fibers immobilize a solute-absorbing phase (preferably organic) which has a greater absorbance affinity towards at least one solute in a mixture of solutes. By passing the solute mixture through the central lumen of the hollow fibers, chromatographic separation are realized due to the greater retention time of that solute with which the immobilized phase has greater absorbance affinity. The immobilized phase may be a liquid organic which may contain a surfactant so as to form reversed micelles or it may be in the form of a polymeric gel. Separations of biological species (e.g., proteins) may thus be accomplished by means of the present invention.

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