Liquid purification or separation – With means to add treating material – Chromatography
Patent
1987-06-17
1988-10-18
Therkorn, Ernest G.
Liquid purification or separation
With means to add treating material
Chromatography
2105021, 210635, 210656, 502401, B01D 1508
Patent
active
047786000
ABSTRACT:
Dual zone reverse phase packing materials for use in liquid chromatographic blood serum analysis are prepared by contacting a porous support, such as porous silica, with a fluorocarbon silane, such as a perfluorobutylsilyl compound, to form a lipophobic phase in the external zone of the porous support, and, then contacting the porous support with a lipophilic silane, such as an octadecylsilyl compound, to form a lipophilic partitioning phase in the internal zone of the porous support. Ketal blocked diol groups, which later may be hydrolyzed to diol groups, are also attached to both zones of the packing materials. The dual zone reverse phase packing materials display a reduced degree of serum protein adsorption in the external zone while drug substances are retained and separated by the lipophilic partitioning phase in the internal zone.
REFERENCES:
patent: 3207699 (1965-09-01), Harding et al.
patent: 3839395 (1974-10-01), Otsuka
patent: 3960720 (1976-06-01), Porath
patent: 3984349 (1976-10-01), Meiller et al.
patent: 4061503 (1977-12-01), Berger et al.
patent: 4062693 (1977-12-01), Berger
patent: 4105465 (1978-08-01), Berger
patent: 4233366 (1980-11-01), Sample et al.
patent: 4298500 (1981-11-01), Abbott
patent: 4324689 (1982-04-01), Shah
patent: 4379931 (1983-04-01), Plueddemann
patent: 4539399 (1985-09-01), Armstrong
patent: 4540486 (1985-09-01), Ramsden
patent: 4544485 (1985-10-01), Pinkerton
patent: 4624932 (1986-11-01), Bogoch
patent: 4675384 (1987-06-01), Dromard
patent: 4696745 (1987-09-01), Itagaki
Chang "High Speed Ion Exchange Chromatography of Proteins" Analytical Chemistry, vol. 48, No. 13, Nov. 1976, pp. 1839-1845.
Poole, C. F., Recent Advances in Silylation of Organic Compounds for Gas Chromatography, Chap. 4, King, Heyden, London 1977, pp. 152-200.
Hertl et al., J. Phys Chem., vol. 75, No. 14, 1971.
Chmielowiec et al., J. of Coll. and Int. Sci, vol. 94, No. 2, Aug. 1983.
Boksanyi et al., Advances in Coll. and Int. Sci., vol. 6 (1976) pp. 95-132.
Snyder et al., Intro. to Modern Leq. Chrom., 2 ed; Wiley Interscience, N.Y. 1979.
Hunnicutt et al., "Reactivity of Organosilane Reagents on Microparticulate Silica", Anal. Chem., vol. 58, 1986, pp. 748-752.
Marshall et al., "Synthesis of LC Reversed Phases of Higher Efficiency by Initial Partial Deactivation of the Silica Surface", Journal of Chrom. Sci., vol. 22, Jun. 1984, pp. 217-220.
Hagestam et al., "Internal Surface Reversed-Phase Silica Supports for Liquid Chromatography," Analytical Chem., vol. 57, 1985, pp. 1757.
Szcerba et al., "HPLC Column Finds Drugs in Serum," Research & Dev., Sep. 1986, pp. 84-86.
Hagestam et al., "Internal Surface Reversed-Phase Silica Support Prepared with Chymotrypsin," Journal of Chrom., vol. 351, 1986, pp. 239-248.
Williams et al., Silica, Surfaces to Interfaces, D. E. Leyden, et al., Gordon to Brench, publishers, 1986, p. 471.ff.
Dow Corning Corporation
Therkorn Ernest G.
LandOfFree
Liquid chromatography dual zone packing materials does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Liquid chromatography dual zone packing materials, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Liquid chromatography dual zone packing materials will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1193678