Liquid chromatography absorbance detector

Optics: measuring and testing – For light transmission or absorption – Of fluent material

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356410, G01N 2101, G01N 2185

Patent

active

047952628

ABSTRACT:
This invention relates to an absorbance detector particularly adapted to liquid chromatography testing. In modern chromatography techniques, light is focused within a small capacity test sample cell, and an optical system is provided to measure the absorbance of various frequencies of light to thereby identify the existence of selected compounds. Inaccuracies of presently available detecting devices result since localized heating within the test cell generates localized index of refraction variations which refract the light in a random fashion such that it is not sensed by the detector. Additional distorting effects are caused by imperfections in the optical surfaces of the system and turbulence due to fluid flow within the sample cell. In accordance with this invention, an appropriate phase conjugator in the form of a retroreflective array is placed in the path of light exiting the sample cell. The array redirects the light rays along their original paths such that they are again distorted but in a reverse sense from the original distortion. In this manner, all of the distorting effects are compensated for. In addition, various means are employed to eliminate the effects of specular reflection from the various optical elements within the system.

REFERENCES:
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patent: 4202600 (1980-05-01), Burke et al.
patent: 4432649 (1984-02-01), Krause
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patent: 4475813 (1984-10-01), Munk
"The Amateur Scientist", Scientific American, published Apr. 1986, by Jearl Walker.
Pittsburgh Conference, presentation with slides, Mar. 1986, Michael D. Morris et al.
"Liquid Chromatography Absorbance Detector with Retroreflective Array for Aberration Compensation and Double Pass Operation", Nov. 1985, T. J. Pang and M. D. Morris.

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