Liquid chromatograph and liquid chromatography

Liquid purification or separation – With means to add treating material – Chromatography

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210656, B01D 1508

Patent

active

058274260

ABSTRACT:
A liquid chromatograph and chromatography which can perform analysis at a higher speed with clearer separation between peaks. The liquid chromatograph comprises a separation column (10), a buffer pump (7) for feeding a plurality of buffers 1, 2, 3 and 4 into the separation column (10), a sampler (9) disposed in a flow path between the buffer pump (7) and the separation column (10) for introducing a sample to be analyzed into the flow path, and a reaction coil (14) for mixing and then reacting amino acids separated by the separation column (10) with a ninhydrin reagent. The separation column is sized so that a ratio (L1/R) of its length L1 to its inner diameter R is not more than 10. The particle size of an ion exchange resin filled in the separation column (10) is selected to be not more than 4 .mu.m.

REFERENCES:
patent: 5236847 (1993-08-01), Satake et al.
patent: 5462659 (1995-10-01), Saxena et al.

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