Liposomes of thermal waters stabilized in a DNA gel

Details Liposomes of thermal waters stabilized in a DNA gel Liposomes of thermal waters stabilized in a DNA gel

1993-06-04

1994-12-27

Kishore, G. S.

Drug, bio-affecting and body treating compositions

Preparations characterized by special physical form

Liposomes

424401, 4284022, A61K 9127

Patent

active

053763793

DESCRIPTION:

BRIEF SUMMARY
The present invention relates to compositions based on thermal water containing liposomes of thermal water stabilized in a DNA gel, which are useful especially in dermatology and in cosmetology. Liposomes are microvesicles consisting of one or more lipid double layers delimiting a central aqueous space and, in the case of multilamellar liposomes, an aqueous behavior between two double layers.
These structures consist of phospholipids to which sterols such as cholesterol are frequently added in order to increase the stability.
Liposomes may be classified according to their size and their uni- or multilammelar character.


______________________________________ MLV (multilammelar vesicles): diameter 100 to 5000 nm (several double layers) LUV (large unilammelar diameter 200 to vesicles): 2000 nm (1 double layer) SUV (small unilammelar diameter 20 to vesicles): 80 nm (1 double layer) ______________________________________
Techniques enabling large quantities of liposomes to be obtained have been developed in industry (ultra-dispersion, sonication, lipopred.) ref. PUISIEUX F., DELATTRE J.--Les liposomes. Application therapeutiques, Technique et Documentation, Lavoisier Paris, 1985.
These microvesicles are capable of interacting with cells whose membranes are identical in nature to that of the liposome.
Dermatology and cosmetology constitute promising sectors for the exploitation of liposomes.
The principal studies relating to the skin relate to the liposomes of corticoids. It would appear that the microencapsulation of corticoids reduces the percutaneous penetration of the product and increases its concentration at local sites : epidermis and dermis. Ref. WOHLRAB W., LASCH J.--Penetration Kinetics of liposomal hydrocortisone in human skin. Dermatologica 174, 18-22, 1987.
Other substances have been incorporated but the studies are too fragmented to be generalized: EGF (Epidermal Growth Factor) is thought to promote the cicatrization of wounds : superoxide dismutases are thought locally to have an anti-inflammatory action. Unfortunately, the liposome vesicles are too fragile in the formulations used and they are often destroyed before reaching their target.
Many others have mentioned the use of gelling agents to stabilize liposomal vesicles in the form of an aqueous gel.
The principal gelling agents used are: gelatin, carboxyvinyl polymers, methacrylic polymers, polydimethyl-siloxane copolymers and, more recently, collagen.
The stabilization of the liposomes in an aqueous gel with the gelling agents currently used does not make it possible to obtain a stability of a formula greater than 3 months at a temperature of 40.degree. C.
The present invention makes it possible to overcome this major disadvantage by stabilizing the liposomes in a deoxyribonucleic acid gel.
According to the invention, the DNA will be DNA which is highly polymerized according to processes known to persons skilled in the art (hereinafter "HP DNA") and which is available commercially.
There may be mentioned in particular DNA having a molecular mass of between 500,000 and 1.5 million, preferably between 800,000 and 1.2 million.
The composition according to the invention advantageously contains 0.1 to 10% by weight of DNA and more particularly 0.5 to 5%.
According to the invention, thermal water, and more particularly AVENE water or CAUTERETS water, is incorporated into the liposomes. AVENE water has therapeutic qualities which are useful in the treatment of eczemas, pruritus, psoriasis, retardation of cicatrization, burns. Basic studies have also supported the effectiveness of AVENE WATER. Thus, several series of studies have made it possible to demonstrate that AVENE WATER exerts an inhibitory effect on the degranulation of human basophiles. It also inhibits the migration of polynuclear cells which have an important role in skin inflammation.
Sulfurated CAUTERETS water, in the form of liposomes, is of interest in dermatology and more particularly in the following treatments: psoriasis, eczemas, acne, pruritus, seborrh

REFERENCES:
patent: 5244672 (1993-09-01), Huc et al.

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