Drug – bio-affecting and body treating compositions – Preparations characterized by special physical form – Liposomes
Patent
1995-03-28
1996-09-03
Rollins, John W.
Drug, bio-affecting and body treating compositions
Preparations characterized by special physical form
Liposomes
435174, 435177, A61K 927, C12N 1100, C12N 1102
Patent
active
055521576
ABSTRACT:
For increasing gene entrapping efficiency, it is preferable that liposome membrane to be formed is positively charged. For this purpose, a lipid with quaternary amine has been employed as one of constitutional lipids, but the such lipid may show toxicity to cells to be transformed with the gene for expression. The invention selects, as constitutional lipids for liposomes, N-(.alpha.-trimethylammonioacetyl)-didodecyl-D-glutamate chloride (TMAG), dilauroylphosphatidylcholine (DLPC), and dioleoylphosphatidylethanolamine (DOPE) with molar ratio of 1:2:2, and vortex treatment for preparing the liposomes with multi-layers to entrap the gene therein.
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Kurono Masayasu
Noda Hitoshi
Ohishi Nobuko
Yagi Kunio
Kabushiki Kaisha Vitamin Kenkyusya
Rollins John W.
Ware Deborah K.
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