Liposome-entrapped DNA oral vaccines

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

Reexamination Certificate

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C424S450000, C435S455000, C514S04400A

Reexamination Certificate

active

07008791

ABSTRACT:
An oral vaccine contain liposomes and complexed or, preferably entrapped, DNA operatively encoding an antigen in which the liposomes are formed from components including cationic compounds and zwitterionic phospholipids. The hydrophobic groups within the liposome forming components must include at least one group which is saturated. This is believed to raise the transition temperature, rendering the liposomes more stable when delivered orally. The compositions have been found to give detectable increased in IgA levels, secreted immunoglobulins of importance in efficacious oral vaccine delivery.

REFERENCES:
patent: 2002/0151070 (2002-10-01), Bischoff et al.
patent: WO 98/10748 (1998-03-01), None
Reyes-Sandoval, Current Molecular Medicine, vol. 1, pp. 217-243, 2001.
Pachuk, Current Opinion in Molecular Therapeutics, 2, 2, 188-198, 2000.
McCluskie et al. (Molecular Medicine, 5, pp. 287-300, 1999).
Gregoriadis et al., “Vaccine Entrapment in Liposomes”, Methods 19 pp. 156-162 (1999).
Perrie et al., “Genetic Immunization Using Liposome-Incorporated DNA”, J. Pharm. Pharmacol. 50 (Supplement) 103 (1998).
Gregoriadis et al., “Liposome-Mediated DNA Vaccination”, FEBS Letters 402, pp. 107-110 (1997).
Han et al., “Application of Liposomes for Development of Oral Vaccines: Study of In Vitro Stability of Liposomes and Antibody Response to Antigen Associated with Liposomes After Oral Immunization” Journal of Veterinary Medical Science vol. 50 No. 12 (Dec. 1997).

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