Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-10-12
1998-08-04
Chin, Christopher L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
422 56, 422 57, 422 58, 4352871, 4352872, 435805, 435810, 435970, 436164, 436169, 436172, 436514, 436518, 436528, 436530, 436533, 436534, 436536, 436538, 436541, 436805, 436807, 436810, 436815, G01N 33543, G01N 33558
Patent
active
057891540
ABSTRACT:
A test device for detecting or determining an analyte in a test solution includes an absorbent material having separate contact, competitive binding, and measurement portions. The contact portion is positioned for contact with and uptake of the test solution. The competitive binding portion has a binding material for the analyte non-diffusively bound thereto. The measurement portion has a receptor for the analyte and marker-encapsulating liposomes non-diffusively bound thereto. In a method for using the test device, a solution containing the analyte and the analyte-liposome conjugate is allowed to traverse the absorbent material from the contact portion through the competitive binding portion and on through the measurement portion of the absorbent material. The amount of marker in the measurement portion of the absorbent material, following traversal by the test solution, is then determined as a measure of the analyte in the sample.
Liposomes encapsulating an electroactive marker are used in conjunction with a test device as described above but which includes an electrochemical measurement portion in place of the measurement portion described above. Test devices and methods employing electrochemical detection or quantification of an electroactive marker corresponding to the amount of analyte in a sample may be either amperometric or potentiometric.
REFERENCES:
patent: 4235601 (1980-11-01), Deutsch et al.
patent: 4517288 (1985-05-01), Giegel et al.
patent: 4517303 (1985-05-01), Freytag et al.
patent: 4594237 (1986-06-01), Zuk
patent: 4636479 (1987-01-01), Martin et al.
patent: 4668619 (1987-05-01), Greenquist et al.
patent: 4703017 (1987-10-01), Campbell et al.
patent: 4752572 (1988-06-01), Sundberg et al.
patent: 4806311 (1989-02-01), Greenquist
patent: 4874710 (1989-10-01), Piran
patent: 4916080 (1990-04-01), Imai et al.
patent: 4920046 (1990-04-01), McFarland et al.
patent: 4939098 (1990-07-01), Suzuki et al.
patent: 5006473 (1991-04-01), Bouma et al.
patent: 5081013 (1992-01-01), Rovelli et al.
patent: 5085987 (1992-02-01), Olson
patent: 5096629 (1992-03-01), Nanba et al.
patent: 5141751 (1992-08-01), Tomikawa et al.
patent: 5308775 (1994-05-01), Donovan et al.
patent: 5310650 (1994-05-01), McMahon et al.
patent: 5354692 (1994-10-01), Yang et al.
patent: 5384264 (1995-01-01), Chen et al.
Locascio-Brown, et al., Liposome Flow Injection Immunoassay: Implications for Sensitivity, Dynamic Range, and Antibody Regeneration, Analytical Chemistry, pp. 2587-2593 (Dec. 1, 1990).
Yap, et al., Liposome Flow Injection Immunoassay: Model Calculations of Competitive Immunoreactions Involving Univalent and Multivalent Ligands, Analytical Chemistry, 63:2007-11 (Sep. 15, 1991).
Durst et al., Automated Liposome-Based Flow Injection Immunoassay System, GBF (Gesellschaft fur Biotechnologische Forschung) Monographs, vol. 14, pp. 181-190 (1990).
Zuk, et al., Enzyme Immunochromatography-A Quantiative Immunoassay Requiring no Instrumentation, Clin. Chem., 31:7, 1144-50 (1985).
Durst, et al., Chemically Modified Electrode for Liposome-Mediated Homogeneous Immunoassay, 5th Symposium on Ion-Selective Electrodes, Pergamon Press, Oxford (1989).
Armbruster, et al., Screening for Drugs of Abuse with the Roche ONTRAK Assays, J. Anal. Tox., vol. 16, pp. 172-175 (May/Jun. 1992).
Losso, et al., Development of a Particle Concentration Flurescence Immunoassay for the Quantitive Determination of IgG in Bovine Milk, J. Agric. Food Chem., vol. 41, pp. 682-686 (1993).
Rosenzweig, et al., Laser-Based Particle-Counting Microimmunoassay for the Analysis of Single Human Erythroytes, Anal. Chem., vol. 66, pp. 1771-1776 (1994).
Collard-Bovy, C., et al., Microparticle-Enhanced Nephelometric Immunoassay. 1. Measurement of .alpha..sub.x -Casein and .kappa..sub.s -Casein, J. Dairy Sci., vol. 74, pp. 3695-3701 (1991).
Heath-Fracia, et al., Evaluation of a New Latex Agglutination Test for Detection of Streptococcal Antibodies, Diagn. Microbiol. Infect. Dis., vol. 8, pp. 25-30 (1987).
Pinnaduwage, et al., Stable Target-Sensitive Immunoliposomes, Biochemistry, vol. 31, pp. 2850-2855 (1992).
Babbitt, et al., Contact-Dependent, Immunecomplex-Mediated Lysis of Hapten-Sensitized Liposomes, Bioconjugate Chem., vol. 4, pp. 199-205 (1993).
Lou, et al., One-Step Competitive Immunochromatographic Assay for Semiquantitative Determination of Lipoprotein(a) in Plasma, Clin. Chem., vol. 39, pp. 619-624 (1993).
Allen, et al., A Nonimstrumented Quantitive Test System and Its Application for Determining Cholesterol Concentration in Whole Blood, Clin. Chem., vol. 36, pp. 1591-1597 (1990).
Zuk, et al., Enzyme Immunochromatography -- A Quantitative Immunoassay Requiring No Instrumentation, Clin. Chem., vol. 31, pp. 1144-1150 (1985).
Plant, et al., Generic Liposome Reagent for Immunoassays, Anal. Biochem., vol. 176, pp. 420-426 (1989).
Monroe, Novel Liposome Immonuassays for Detecting Antigens, Antibodies and Haptens, J. Liposome Res., vol. 1, pp. 339-377 (1989 (1989-90).
Durst Richard Allen
Reeves Stuart Graham
Siebert Sui Ti Atienza
Chin Christopher L.
Cornell Research Foundation Inc.
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