Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1995-06-06
1998-05-19
Chin, Christopher L.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
4272133, 42721334, 42721335, 2041827, 204286, 204288, 204290R, 204297R, 435 3, 435 71, 435 793, 435970, 422 56, 422 57, 422 70, 422 8201, 422 8203, 422 98, 422 99, 422110, 436516, 436530, 436541, 436514, G01N 3353, C25B 100, B01J 1303
Patent
active
057535191
ABSTRACT:
A test device for detecting or quantifying an analyte in a test sample includes an absorbent material having separate contact and measurement portions. The contact portion is positioned at or proximate to a first end of the absorbent material. The measurement portion has a receptor for a conjugate of an analyte analog and marker-encapsulating liposomes. In a method for using the test device, a binding material specific for the analyte is combined with the liposome-analyte analog conjugate and the test sample to form a test mixture. The mixture is incubated for a time sufficient to permit competition between any analyte present and the conjugate for the binding material. Following incubating, the mixture is allowed to traverse the absorbent material from the contact portion through the measurement portion of the absorbent material. Following traversal by the test mixture, the presence or amount of marker in the measurement portion of the absorbent material is then detected and correlated with the presence or amount, respectively, of the analyte in the sample. Also disclosed are liposomes encapsulating an electroactive marker used in conjunction with a test device as described, but which includes an electrochemical measurement portion in place of the measurement portion described above. Test devices and methods employing electrochemical detection or quantification of an electroactive marker corresponding to the amount of analyte in a sample may be either amperometric or potentiometric.
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Durst Richard Allen
Roberts Matthew A.
Chin Christopher L.
Cornell Research Foundation Inc.
Nguyen Bao-Thuy L.
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