Lipoprotein diluent or solution and method useful in the prepara

Details Lipoprotein diluent or solution and method useful in the prepara Lipoprotein diluent or solution and method useful in the prepara

1978-05-15

1980-08-05

Turk, Arnold

Compositions

Nonreactive analytical, testing, or indicating compositions

23230B, G01N 3100, G01N 3316

Patent

active

042161177

ABSTRACT:
A lipoprotein diluent useful in the preparation of a standard or reference material for assay procedures which comprises a stabilized aqueous solution of turbidity-potential lipoproteins. The solution contains a preservative including a polyhydroxy cryoprotective agent and/or an antibiotic, and may be used directly as a cholesterol or triglyceride standard or control. The solution may also include other stable components but is free from lipoprotein-degrading enzymes. In accordance with the method of the present invention, a standard or reference material is prepared by adding a diluent containing turbidity-potential lipoproteins to a lyophilized serum base, which contains enzymes.

REFERENCES:
patent: 3260648 (1966-07-01), Fox
patent: 3632735 (1972-01-01), Kita et al.
patent: 3753925 (1973-08-01), Louderback et al.
patent: 3764556 (1973-10-01), Kuchmak et al.
patent: 3955925 (1976-05-01), Proksch et al.
patent: 4045176 (1977-08-01), Proksch et al.
patent: 4127502 (1978-11-01), Mutti et al.
Bernfeld et al., "The Influence of Chem. & Physicochemical Nature of Macromolecular Polyanions on Their Interaction with Human Serum .beta.-Lipoproteins," Journal of Biological Chemistry; Oct. 1960; vol. 235; No. 10; pp. 2852-2859.
Oncley et al.; "A Rapid Method for the Bulk Isolation of .beta.-Lipoproteins from Human Plasma"; Journal of the Amer. Chem. Soc.; vol. 79, No. 13, Jul. 5, 1957; pp. 4666-4671.

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