Lipopolysaccharides of reduced toxicity and the production there

Details Lipopolysaccharides of reduced toxicity and the production there Lipopolysaccharides of reduced toxicity and the production there

1991-07-08

1993-04-06

Patterson, Jr., Charles L.

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Hydrolases

435187, 514 53, A61K 3754, A61K 31715, C12N 918

Patent

active

052001849

ABSTRACT:
An acyloxyacyl hydrolase from the human promyelocyte cell line HL-60 has been found to specifically hydrolyze fatty acids from their ester linkages to hydroxy groups of 3-hydroxyfatty acids, the latter being bound in turn to LPS glycosaminyl residues. The hydrolyzed fatty acids may include dodecanoic acid, tetradecanoic acid and hexadecanoic acid. This enzyme showed a molecular weight by gel exclusion chromatography between about 50,000 Daltons and about 70,000 Daltons.
Altered bacterial LPS substantially without fatty acids bound in ester linkage to hydroxy groups of 3-hydroxyfatty acids covalently linked to a glucosaminyl moiety of LPS lipid A are produced. Since the structure of the lipid A moiety is highly conserved, ac

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