Lipopolysaccharide binding protein and process for producing the

Details Lipopolysaccharide binding protein and process for producing the Lipopolysaccharide binding protein and process for producing the

1995-02-23

1998-06-02

Tsang, Cecilia J.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

5303895, 530396, 530402, 530413, 530416, 530417, 530427, 530820, 4241931, 4241941, 424520, 424547, C07K 1400, A61K 39385

Patent

active

057601771

ABSTRACT:
The invention provides a lipopolysaccharide (LPS) binding protein isolated from horseshoe crab. The LPS binding protein is isolated by (i) extracting the hemocyte membrane fraction of horseshoe crab with a polyethylene glycol ether type nonionic surface active agent in the presence of Ca ions, (ii) combining the extract with immobilized LPS under conditions that permit the LPS binding protein to bind the immobilized LPS to produce an LPS-LPS binding protein complex, and (iii) harvesting the LPS binding protein released from the complex in the presence of a chelating agent. The isolated LPS binding protein has a molecular weight of about 27,000 daltons as determined by SDS polyacrylamide gel electrophoresis and is operative to bind a lipopolysaccharide endotoxin. Accordingly, the isolated LPS binding protein can be used for detecting endotoxin and/or removing endotoxin from an injectable medicine.

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