Lipophilic fluorescent glycosidase substrates

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 19, 435 79, 435 793, 536 41, 536 181, C07H 1500, C08B 3702, G01N 3354, C12Q 134

Patent

active

052081486

ABSTRACT:
The claimed invention relates to a substrate for evaluating glycosidic enzymes comprising a fluorescein derivative of the general formula: ##STR1## wherein GlyX is a carbohydrate bonded to fluorescein by a glycosidic linkage;

REFERENCES:
patent: 4193983 (1980-03-01), Ullman et al.
patent: 4268663 (1981-05-01), Skold
patent: 4279992 (1981-07-01), Boguslaski et al.
patent: 4812409 (1989-03-01), Babb et al.
Chemical Abstracts vol. 115 (11): 109263g (1991).
Chemical Abstracts vol. 114 (5): 38651y (1990).
Chemical Abstracts vol. 105 (21): 186354h (1986).
Chemical Abstracts vol. 97 (16): 133318j (1982).
Chemical Abstracts vol. 84 (19): 133977e (1976).
Chemical Abstracts vol. 116 (13): 122197d (1991).
Rotman, et al., "Fluorogenic Substrates for Beta-D-galactosidases and Phosphotases Derived from Fluorescein (3,6-Dihydroxyfluoran) and Its Monomethyl Ether," Proc. Nat. Acad. Sci. 50:1 (1963).
Jefferson, "The GUS Reporter Gene System," Nature 342:837 (1989).
Enzyme Nomenclature pp. 306-326 (Int'l Union Biochem., Academic Press 1984).
Jarvis, et al., "Identification of a DNA segment that is necessary and sufficient for .alpha.-specific gene control in Saccharomyces cerevisiae: implications for regulation of .alpha.-specific and a-specific genes," Molec. & Cell Biol. 8:309 (1989).
Legler et al., "Glucosylceramidase from Calf Spleen," Biol. Chem. 366:1113 (1989).
Galbraith, "Selection of Somatic Hybrid Cells by Fluorescence-Activated Cell Sorting," Cell Culture and Somatic Cell Genetics of Plants 1:433, ch. 50 (1984).
Nolan, et al., "Fluorescence-activated cell analysis and sorting of viable mammalian cells based on .beta.-D-galactosidase activity after transduction of Escherichia coli lacZ," Cell Biol. 85:2603 (1988).
Ikenaka, et al., "Laboratory Methods: Reliable Transient Promoter Assay Using Fluorescein-di-.beta.-D-galactopyranoside Substrate," DNA & Cell Biol. 9:279 (1990).
Steinbach, "Characterization of fluorescein isothiocyanate: Synthesis and testing methods for fluorescein isothiocyanate isomers [sic]," Acta Histochem. 49:19 (1974).
Emilie et al., "Visualizing interleukin 2 gene expression at the single cell level," Eur. J. Immunol. 19:1619 (1989).
Stryer, Biochemistry p. 189, W. H. Freeman & Co. Editors (1988).
Jefferson et al., "GUS Fusions: .beta.-Glucuronidase as a sensitive and versatile gene fusion marker in higher plants," EMBO J. 6:3901-07 (1987).
Naleway, et al., "New .beta.-Galactosidase Substrates with Improved Cell Retention and Loading Properties in LacZ Positive Cells," J. Cell Biol. 111:380a para. 2132 (Nov. 1990).
Petushkova, et al. Study of Certain Properties of .beta.-Galactocerebrosidase of the Human Chorion Using a Synthetic Fluorogenic Substrate, translation reprinted from Biokhimya 53, 1539.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Lipophilic fluorescent glycosidase substrates does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Lipophilic fluorescent glycosidase substrates, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Lipophilic fluorescent glycosidase substrates will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1974731

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.