Cleaning compositions for solid surfaces – auxiliary compositions – Cleaning compositions or processes of preparing – For cleaning a specific substrate or removing a specific...
Reexamination Certificate
2007-09-18
2007-09-18
Saidha, Tekchand (Department: 1652)
Cleaning compositions for solid surfaces, auxiliary compositions
Cleaning compositions or processes of preparing
For cleaning a specific substrate or removing a specific...
C426S020000, C435S198000, C536S023200
Reexamination Certificate
active
11474151
ABSTRACT:
The inventors have isolated novel lipolytic enzyme genes with a high homology to theT. lanuginosuslipase gene and thus well suited for use in gene shuffling. Accordingly, the invention provides a method of generating genetic diversity into lipolytic enzymes by family shuffling of two or more homologous genes which encode lipolytic enzymes. The DNA shuffling technique is used to create a library of shuffled genes, and this is expressed in a suitable expression system and the expressed proteins are screened for lipolytic enzyme activity. The invention also provides a polynucleotide comprising a nucleotide sequence encoding a lipolytic enzyme and a lipolytic enzyme (a polypeptide with lipolytic enzyme activity).
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Sequence search alignment between Applicants' SEQ ID No. 6 and Accession No. AAR22639 in WO92/05249.
Sequence search alignment between Applicants' SEQ ID No. 6 and mature lipase (AAO19510) from Thermomyces ibadanensis in WO200262973-A2.
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Anant Patkar Shamkant
Tsutsumi Noriko
Vind Jesper
Garbell Jason I.
Novozymes A/S
Saidha Tekchand
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