Lipidating sequences and use thereof for producing lipidated...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Reexamination Certificate

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C435S320100, C435S069700, C435S069800, C435S071100, C536S023400, C536S023700

Reexamination Certificate

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07833776

ABSTRACT:
Production inE. coliof a lipidated fusion protein containing a lipidating sequence derived from Ag473 and a target polypeptide.

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Legrain et al., “Production of Lipidated Meningococcal Transferrin Binding Protein 2 inEscherichia coli” Protein Expression and Purification 6:570-578 (1995).
De et al., “Purification and Characterization ofStreptococcis pneumoniaepalmitoylated and pneumococcal surface adhesion A expressed inEscherichia coli,” Vaccine: 18: 1811-1821 (2000).
Cullen et al., “Construction and Evaluation of a Plasmid Vector for the Expression of Recombinant Lipoproteins inEscherichia coli,” Plasmid 49: 18-29 (2003).
Kamalakkannan et al., “Bacterial Lipid Modification of Proteins for Novel Protein Engineering Applications,” Protein, Engineering, Design & Selection 17(10): 721-729 (2004).

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