Lipid structures and uses thereof

Details Lipid structures and uses thereof Lipid structures and uses thereof

2006-06-13

2006-06-13

Lankford, Jr., Leon B. (Department: 1651)

Drug, bio-affecting and body treating compositions

Preparations characterized by special physical form

Liposomes

C424S001110, C424S001210, C436S063000, C436S172000, C435S317100

Reexamination Certificate

active

07060292

ABSTRACT:
This invention features in vitro methods of determining the rate and extent to which test compounds, such as drug candidates, enter, move within, and traverse lipid-associated barriers. This invention also features method that measure the accumulation, that is the partition coefficient, of test compounds in lipid structures relative to, e.g., an aqueous phase, as well as the rate of accumulation of test compounds in the lipid structures. In addition, the invention features lipid vesicles that contain fluorophores localized within the aqueous interior that can be employed in the methods described herein.

REFERENCES:
patent: 5094819 (1992-03-01), Yager et al.
patent: 5106610 (1992-04-01), Harris et al.
patent: 5709994 (1998-01-01), Pease et al.
patent: 6063589 (2000-05-01), Kellogg et al.
patent: 6107066 (2000-08-01), Tsien et al.
patent: 6235535 (2001-05-01), Keinanen et al.
patent: 6251581 (2001-06-01), Ullman et al.
patent: 6302134 (2001-10-01), Kellogg et al.
patent: 6342379 (2002-01-01), Tsien et al.
patent: WO 98/37920 (1998-02-01), None
Alberts et al “Molecular Biology of the Cell” 1994, Garland Publishing, Inc, 3rd Edition, pp. 480-483.
Carruthers et al, Biochemistry, 1984, vol. 23, pp. 6901-6911.
Barton et al. “Drug-Phospholipid Interactions. 2. Predicting the Sites of Drug Distribution Usingn-Octanol/Water and Membrane/Water Distribution Coefficients”J. Pharm. Sci. 86: 1034-1039 (1997).
Carruthers et al. “A Rapid Method of Reconstituting Human Erythrocyte Sugar Transport Proteins”Biochemistry23: 2712-2718 (1984).
Fix et al. “The Fluorosome™ Technique for Investigating Membrane On- and Off-Loading of Drugs by β-CD and Sonicated SUV”FEBS Lett. 516: 109-112 (2002).
Gobas et al. “A Novel Method for Measuring Membrane-Water Partition Coefficients of Hydrophobic Organic Chemicals: Comparison with 1-Octanol-Water Partitioning”J. Pharm. Sci. 77: 265-272 (1988).
Hsu et al. “Fatty Acid Transfer from Liver and Intestinal Fatty Acid-binding Proteins to Membranes Occurs by Different Mechanisms”J. Biol. Chem. 271:13317-13323 (1996).
Kansy et al. “Physicochemical High Throughput Screening: Parallel Artificial Membrane Permeation Assay in the Description of Passive Absorption Processes”J. Med. Chem. 41: 1007-1010 (1998).
Levine et al. “Structural and Physiologic Determinants of Human Erythrocyte Sugar Transport Regulation by Adenosine Triphosphate”Biochemistry37: 12221-12232 (1998).
Melchior et al. “Application of Fluorosome Stopped-Flow Spectrophotometry to Monitor the Entry of Molecules into Lipid Bilayers”Biotechnol. Tech. 11: 885-888 (1997).
Melchior “A Rapid Empirical Method for Measuring Membrane Bilayer Entry Equilibration of Molecules”J. Pharm. Sci. 91: 1075-1079 (2002).
Noy, et al. “Kinetic Parameters of the Interactions of Retinol with Lipid Bilayers”Biochemistry29: 3883-3888 (1990).
Ottiger et al. “Partition Behaviour of Acids and Bases in a Phosphatidylcholine Liposome-Buffer Equilibrium Dialysis System”Eur. J. Pharm. Sci. 5: 223-231 (1997).
Pauletti et al. “Partition Coefficients in vitro: Artificial Membranes as a Standardized Distribution Model”Eur. J. Pharm. Sci. 1: 273-282 (1994).
Sarmento et al. “Partition of Dopamine Antagonists into Synthetic Lipid Bilayers: The Effect of Membrane Structure and Composition”J. Pharm. Pharmacol. 45: 601-605 (1993).
Verkman “The Quenching of an Intramembrane Fluorescent Probe. A Method to Study the Binding and Permeation of Phloretin through Bilayers”Biochim. Biophys. Acta599: 370-379 (1980).

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