Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Conjugate or complex
Patent
1981-12-16
1984-12-18
Friedman, Stanley J.
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Conjugate or complex
A61K 3578
Patent
active
044890672
DESCRIPTION:
BRIEF SUMMARY
BACKGROUND AND FIELD OF THE INVENTION
The present invention relates to lipid reducing agents and more specifically to those agents which reduce the amount of lipids in serum and in the liver, said agents comprising substances solvent-extracted from Yucca plants.
Diseases caused by the accumulation of lipids in serum and in the liver have become a serious concern in recent years, and drugs which can alleviate these diseases have been much desired.
DISCLOSURE OF THE INVENTION
As a result of extensive research in connection with attempts to collect from Yucca plants substances which can reduce the amount of lipids in serum and in the liver, the inventors have attained the present invention.
According to the present invention, substances having a function of reducing the amount of lipids in serum and in the liver can be separated from Yucca plants and refined by the following two exemplary methods.
(1) The stems and rootstocks of Yucca plants are dried and powdered. The powder is then dissolved in a lower dialkyl ether such as ethyl ether to remove an ether-soluble portion and the residue is extracted with a lower alcohol. From the extract, the lower alcohol is distilled off and the residue is dried. This residue is used as an effective component and thus "lipid reducing agent I" is prepared.
(2) The stems and rootstocks of Yucca plants are dried and powdered. The powder is dissolved in a lower dialkyl ether such as ethyl ether to remove an ether-soluble portion. The residue is extracted with a lower alcohol and the extract is concentrated to obtain an extract. The extract is again extracted with water-saturated ethyl acetate and, from the extract, the water-saturated ethyl acetate is distilled off. The remaining fluid is adsorbed on silica gel and the adsorbed substances are eluted from the silica gel in two steps, namely in the first step using a mixed organic solvent consisting of a major proportion, e.g., eight volume parts of chloroform and a minor proportion, e.g., one volume part of a lower alcohol such as methyl alcohol and in the second step using a mixed organic solvent consisting of a major proportion, e.g., four volume parts of chloroform and a minor porportion, e.g., one volume part of a lower alcohol such as methyl alcohol. From the second eluate, the solvent is distilled off and the residue is dried. This residue is used as an effective component and thus "lipid reducing agent II" is prepared.
In the present invention, a lower alcohol such as methyl alcohol, ethyl alcohol or isopropyl alcohol can be used for extraction purposes.
The following examples are given merely as illustrative of the present invention and are not to be considered as limiting. Unless otherwise noted, all percentages therein and throughout the application are by weight.
EXAMPLE 1
Preparation of lipid reducing agent I
Stems and rootstocks of Yucca mohavensia were dried and powdered. One kilogram of the powder was dipped in 4 liters of ethyl ether for about 2 hours at room temperature. The resultant ethyl ether extract was removed. Then, to the ether-insoluble portion was added 4 liters of methyl alcohol. The mixture was refluxed with heating for 3 hours for extraction and then filtered after cooling. To the insoluble portion was added an additional 3.5 liters of methyl alcohol and a similar procedure was repeated. The two extracts were mixed and concentrated under reduced pressure to remove the methyl alcohol, and about 400 ml of a concentrated fluid was obtained. By drying the fluid, 324 g of a dried substance was obtained.
EXAMPLE 2
Preparation of lipid reducing agent II
Stems and rootstocks of Yucca mohavensia were dried and powdered. One kilogram of the powder was dipped in 4 liters of ethyl ether for about 2 hours at room temperature. The resultant ether extract was removed. Then, to the remaining ether-insoluble portion was added 4 liters of methyl alcohol and the mixture was refluxed with heating for 3 hours for extraction. After cooling, the mixture was filtered. To the methyl alcohol-insoluble fracti
Matsuura Shin
Shioyama Masakazu
Uenobe Fukuji
Yamamoto Nobuhiro
Friedman Stanley J.
Rollins Jr. John W.
Yushiro Chemical Industry Co., Ltd.
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