Lipid-encapsulated polyanionic nucleic acid

Details Lipid-encapsulated polyanionic nucleic acid Lipid-encapsulated polyanionic nucleic acid

2005-02-22

2005-02-22

Naff, David M. (Department: 1651)

Drug, bio-affecting and body treating compositions

Preparations characterized by special physical form

Liposomes

C428S402200, C435S177000, C435S458000, C514S04400A, C536S022100

Reexamination Certificate

active

06858225

ABSTRACT:
Methods for the preparation of a lipid-nucleic acid composition are provided. According to the methods, a mixture of lipids containing a protonatable or deprotonatable lipid, for example an amino lipid and a lipid such as a PEG- or Polyamide oligomer-modified lipid is combined with a buffered aqueous solution of a charged therapeutic agent, for example polyanionic nucleic acids, to produce particles in which the therapeutic agent is encapsulated in a lipid vesicle. Surface charges on the lipid particles are at least partially neutralized to provide surface-neutralized lipid-encapsulated compositions of the therapeutic agents. The method permits the preparation of compositions with high ratios of therapeutic agent to lipid and with encapsulation efficiencies in excess of 50%.

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