Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving blood clotting factor
Patent
1986-02-19
1987-10-06
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving blood clotting factor
435 7, 436175, 436506, 436511, 436825, G01N 33564, G01N 33571, G01N 3396
Patent
active
046982990
ABSTRACT:
Lipid-dependent diagnostic assays are provided wherein the test sample to be assayed is pre-incubated with one or more phospholipids having a hexagonal (H.sub.II) organization, with lipidic particles, or with bilayer-forming lysophospholipids such as monooleoylphosphatidylethanolamine (MOPE). The pre-incubation results in reduced false positives due to antiphospholipid antibodies, such as, lupus anticoagulants, which may be present in the test sample, without changing the overall character, including the normal baseline, of the assay. Surprisingly, in accordance with the invention, it has been found that only hexagonal phospholipids, lipidic particles, and bilayer-forming lysophospholipids can be used, and, in particular, lamellar (bilayer) phospholipids (other than such lysophospholipids) cannot be used. An assay for diagnosing systemic lupus erythematosus (SLE) is also provided.
REFERENCES:
patent: 3564089 (1971-02-01), Kiddy
patent: 3600494 (1971-08-01), Tomizawa
patent: 4076797 (1978-02-01), Davis
patent: 4273867 (1981-06-01), Lin
Boey, M. L. et al., "Thrombosis in Systemic Lupus Erythematosus: Striking Association With the Presence of Circulating Lupus Anticoagulant," British Med. Journ., 287, pp. 1021-1023, 1983.
Margolius, A. et al., "Circulating Anticoagulants: A Study of 40 Cases and a Review of the Literature," Medicine, 40, pp. 145-202, 1961.
Lafer, E. M. et al., "Polyspecific Monoclonal Lupus Autoantibodies Reactive With Both Polynucleotides and Phospholipids", Journal Experimental Medicine, 153, pp. 897-909, 1981.
Folch, J. "Brain Cephalin, a Mixture of Phosphatides, Separation from it of Phosphatidyl Serine, Phosphatidyl Ethanolamine & a Fraction Containing an Inositol Phosphatide," J. Biol. Chem., 146, pp. 35-44, 1942.
Exner, T. et al., "Studies on Phospholipids in the Action of a Lupus Coagulation Inhibitor," Pathology, 7, pp. 319-328, 1975.
Thiagarajan, P., et al., "Monoclonal Immunoglobulin M.lambda. Coagulation Inhibitor With Phospholipid Specificity," J. Clin. Invest., 66, pp. 397-405, 1980.
Sparling, P. "Diagnosis and Treatment of Syphilis", New England Journal of Medicine, 284, pp. 642-653, 1971.
Janoff Andrew S.
Rauch Joyce
Tilcock Colin P. S.
Bloom Allen
Kurtz Catherine L.
Marantz Sidney
The Liposome Company Inc.
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