Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving blood clotting factor
Patent
1985-02-19
1987-05-19
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving blood clotting factor
436 63, 436 69, 436175, 436506, 436511, 436811, 436825, G01N 3353, G01N 33571
Patent
active
046668314
ABSTRACT:
Improved lipid-dependent diagnostic assays are provided wherein the test sample to be assayed is pre-incubated with one or more phospholipids having a hexagonal (H.sub.II) organization or with lipidic particles. The pre-incubation results in reduced false positives due to anti-phospholipid antibodies, such as, lupus anticoagulants, which may be present in the test sample, without changing the overall character, including the normal baseline, of the assay. Suprisingly, in accordance with the invention, it has been found that only hexagonal phospholipids and lipidic particles can be used, and, in particular, lamellar (bilayer) phospholipids cannot be used. An assay for diagnosing systemic lupus erythematosus (SLE) is also provided.
REFERENCES:
patent: 3564089 (1971-02-01), Kiddy
patent: 3600494 (1971-08-01), Tomizawa
patent: 4076797 (1978-02-01), Davis
patent: 4273867 (1981-06-01), Lin
Exner et al., Pathology, vol. 7, pp. 319-328, 1975.
Thiagarajan et al., J. Clin. Invest., vol. 66, pp. 397-405, 1980.
Sparling, P. F., New England Journal of Medicine, vol. 284, pp. 642-653, 1971.
Folch, J., J. Biol. Chem., vol. 46, pp. 35-41, 1942.
Cullis et al., Membrane Fluidity in Biology, vol. 1, Academic Press, Chapter 2, pp. 39-81, 1983.
Janoff Andrew S.
Rauch Joyce
Tilcock Colin P. S.
Marantz Sidney
The Liposome Company Inc.
LandOfFree
Lipid-dependent diagnostic assays does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Lipid-dependent diagnostic assays, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Lipid-dependent diagnostic assays will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1564636