Lipases from thermophilic anaerobes

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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C435S197000, C435S198000, C435S018000, C435S019000, C536S023200

Reexamination Certificate

active

07972831

ABSTRACT:
The inventors have identified polypeptides having lipase activity in anaerobic thermophilic bacteria. Accordingly, the invention provides a process for hydrolyzing an ester bond in a substrate, which comprises treating the substrate with a particular lipase (a polypeptide having lipase activity). The invention also provides a lipase for use in the process and a polynucleotide encoding the lipase.

REFERENCES:
patent: 6537787 (2003-03-01), Breton
patent: 1500868 (2004-06-01), None
EMBL/UniProt Accession No. Q8R921, Hydrolase of the alpha/beta superfamily, Jun. 1, 2002.
Whisstock et al. Quaterly Reviews of Biophysics, 2003, “Prediction of protein function from protein sequence and structure”, 36(3): 307-340.
Bao et al., Genome Research , vol. 12, pp. 689-700 (2002).
EMBL: AEO13133, Accession No. AEO13133 (May 12, 2002).
Nolling et al., Journal of Bacteriology, vol. 183, No. 16, pp. 4823-4838 (2001).
EMBL: AE007861, Accession No. AE007861 (Jul. 31, 2001).
Rogalska et al., Chirality, vol. 5, pp. 24-30 (1993).
International Search Report from PCT/DK2005/000639, Parent PCT filed on Oct. 5, 2005.

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