Lipase, microorganisms producing the lipase, method of producing

Details Lipase, microorganisms producing the lipase, method of producing Lipase, microorganisms producing the lipase, method of producing

1996-03-14

1998-10-27

Hutzell, Paula K.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435183, 435188, 435196, 435264, 435874, 25217412, 530350, 530825, 4242601, 424 941, C12N 920

Patent

active

058277188

DESCRIPTION:

BRIEF SUMMARY
This application is a 371 of PCT/JP94/0146 filed Aug, 26, 1994.


TECHNICAL FIELD

This invention relates to a novel lipase, to microorganisms producing the lipase, to a method of producing the lipase, and to use of the lipase. More particularly, this invention relates to the lipase which bacteria belonging to the genus Pseudomonas produce and which has activity in washing liquids (in alkaline regions), to microorganisms producing the lipase, to a method of producing the lipase, and to detergent compositions containing an enzyme or enzymes which can decompose lipids in the washing liquids.


BACKGROUND ART

It has been known to utilize enzymes for blending them with detergents in order to improve the washing efficiency in washing. For example, it has been known to blend proteases with detergent compositions to decompose and remove proteins and other dirt on articles to be washed, to blend cellulases with detergent compositions to remove dirt on cellulose fabrics to be washed, or to blend polysaccharide decomposing enzymes such as amylase to decompose and remove polysaccharides and other dirt attached on articles to be washed. In addition, recently, it has been known that blending lipases with detergents can decompose and remove lipids on articles to be washed so that the washing efficiency can be increased. This use is described by H. Andree et al., in the report "Lipase as detergent components," Journal of Applied Biochemistry, 2, 218-229 (1980) and so on.
Preferred lipases for detergents are those which exhibit their lipase activities sufficiently in washing liquids. Under ordinary washing conditions, the pH of the washing liquid is in alkaline range and, hence, lipases are required which function in alkaline pH range. Generally, lipid dirt is known to be relatively easy to remove under the conditions of high temperature and high alkalinity while it is impossible to sufficiently remove by washing at low temperatures (not higher than 60.degree. C.). Not only in Japan where washing is being done mainly at lower temperatures but also in European countries and the United States, washing temperatures tend to be decreased. Therefore, preferred lipases for detergents must function sufficiently even at low temperatures. In addition, preferred lipases for detergents should exhibit their function upon washing even in the presence of detergent components such as surfactants as well as proteases and bleaching agents as contained in many detergents. Furthermore, preferred lipases for detergents, when they are stored in a state blended in detergents, should be stable against components copresent in the detergents. Accordingly, there is a desire for development of detergent compositions which contain lipases with the above-described preferred features and have high washing effect against lipid dirt.
Lipases produced by microorganisms include those derived from microorganisms belonging to the genera Pseudomonas, Alcaligenes, Achromobacter, Mucor, Candida, Humicola, etc.
However, most lipases obtained from these strains have their optimum pH in the regions of from neutral to weak alkaline so that they do not function sufficiently in detergent solutions in alkaline regions and show low stability in the detergent solutions.
Further, the respective lipases derived from the microorganisms belonging to the genera Achromobacter, Candida, Mucor, and Humicola suffer strong inhibition of their activities in the presence of anionic surfactants.
It has been widely known that microorganisms belonging to the genus Pseudomonas produce lipases. The strains of the genus Pseudomonas include Ps. fluorescens, Ps. cepacia, Ps. fragi, Ps. alcaligenes, and Ps. pseudoalcaligenes, Ps. aeruginosa. The known lipases obtained from these strains do not satisfy the above-described characteristics.


OBJECT OF THE INVENTION

Accordingly, an object of this invention is to provide a lipase which functions sufficiently in washing liquids, whose activity is not inhibited substantially by components copresent in the detergent, and which shows high stabili

REFERENCES:
patent: 4933287 (1990-06-01), Farin
patent: 5059341 (1991-10-01), Holmes
patent: 5063160 (1991-11-01), Holmes
Hans Andree, Wolf-R Muller and Rolf D. Schmid, Lipases as Detergent Components. J. Appl. Biochem. 2, 218-229 (1980).

Affiliated with

Also associated with

Rating

Lipase, microorganisms producing the lipase, method of producing does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Lipase, microorganisms producing the lipase, method of producing, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Lipase, microorganisms producing the lipase, method of producing will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1613213