Lipase immobilized with hydrophilized polyolefin fibers for reso

Details Lipase immobilized with hydrophilized polyolefin fibers for reso Lipase immobilized with hydrophilized polyolefin fibers for reso

1988-11-04

1992-04-07

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435134, 435135, 435177, 435182, 435198, 435280, C12N 1108, C12N 920, C12P 764, C12P 762

Patent

active

051027992

ABSTRACT:
Immobilized lipase is prepared by forming a mixture of hydrophilized polyolefin fibers, crude porcine pancreas lipase, one or more water soluble dihydric to hexahydric aliphatic alcohols of 2 to 6 carbons and an aqueous buffer solution of pH 5-9 at a temperature of 0.degree. to 40.degree. C., allowing the mixture to stand for 1 minute to 3 days, filtering the mixture and washing the resultant filter cake. The dihydric to hexahydric alcohol may be combined with one or more water soluble monohydric alcohols having 1 to 4 carbon atoms. The immobilized lipase is used for resolving racemates of esters of racemic alcohols in an aqueous medium.

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Journal of American Chemical Society 1984, 106, pp. 7250-7251, Lipase-Catalysed Hydrolysis as a Route to Esters of Chiral Epoxy Alcohols Wolfgang Ladner, et al.

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