Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1994-08-11
1996-04-16
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
435174, C12N 1100, C12N 1110
Patent
active
055081851
ABSTRACT:
An enzyme immobilizing carrier is produced by dissolving low-molecular weight chitosan in an aqueous acid solution and dropping the solution into a basic solution to produce regenerated porous chitosan in particles, reacting the regenerated porous chitosan in particles with the glycidyl ether of an aliphatic polyalcohol, and reacting further the resulting chitosan with the acid halide or acid anhydride of a higher fatty acid in a polar organic solvent. An enzyme and a polyfunctional cross-linking agent are reacted with the carrier to covalently immobilize the enzyme on the carrier. In a preferred embodiment, the enzyme is lipase and the carrier is produced by introducing the glycidyl ether of an aliphatic polyalcohol at 0.01 to 0.4 mole to 1 mole of the pyranose ring residue of the chitosan and by introducing a higher fatty acid having a total carbon number of 6 to 20 at 0.05 to 1 mole to 1 mole of the pyranose ring residue of the chitosan.
REFERENCES:
"Immobilized Lipase Reactors for Modification of Fats and Oils--A Review", Journal of American Oil Chemist's Society, vol. 67, No. 12 (Dec. 1990).
Harada Junko
Imamura Shigeyuki
Kawamura Yoshihide
Tanibe Hiroaki
Fuji Spinning Co., Ltd.
Naff David M.
Ware Deborah K.
LandOfFree
Lipase immobilized on a chitosan carrier does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Lipase immobilized on a chitosan carrier, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Lipase immobilized on a chitosan carrier will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-324519