Lipase immdoilized by cross-linking with inert protein for glyce

Details Lipase immdoilized by cross-linking with inert protein for glyce Lipase immdoilized by cross-linking with inert protein for glyce

1988-08-11

1991-04-23

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435134, 435174, C12N 1102, C12N 1100, C12P 764

Patent

active

050100068

ABSTRACT:
A dry porous macromolecular matrix is prepared comprising a cross-linked mixture of 69.9 to 86.6% by weight of an inert protein 0.4 to 10.2% by weight of a lipase enzyme and about 11.7 to 23% by weight of a cross-linking agent. The matrix is prepared by reacting the lipase, inert protein and cross-linking agent in a buffer solution, freezing the resulting reacted mixture at a temperature ranging between -20.degree. C. and -195.degree. C., allowing the frozen mixture to thaw to a temperature ranging from 4.degree. C. to 25.degree. C., and rinsing and drying the resulting product at room temperature.

REFERENCES:
patent: 3705084 (1972-12-01), Reynolds
patent: 3796634 (1974-03-01), Haynes et al.
patent: 4464468 (1984-08-01), Arrameas et al.
patent: 4472503 (1984-09-01), Matsuo et al.
patent: 4798793 (1989-01-01), Eigtred
G. Broun et al., "New Methods for Binding Enzyme Molecules into a Water-Insoluble Matrix: Properties after Insolubilization", Biotechnology and Bioengineering, vol. XV, pp. 359-375 (1973).

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