Lipase from Pseudomonas mendocina having cutinase activity

Details Lipase from Pseudomonas mendocina having cutinase activity Lipase from Pseudomonas mendocina having cutinase activity

1991-05-23

1995-02-14

Knode, Marian

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435195, 4351723, 935 14, 930240, C12N 920

Patent

active

053895363

ABSTRACT:
A substantially enzymatically pure hydrolase is provided which is secreted by and isolatable from Pseudomonas mendocina ATCC 53552. Cloning the gene expressing the hydrolase into a suitable expression vector and culturing, such as fermenting the E. coli strain JM101 harboring a plasmid designated pSNtacII, has been found to provide surprisingly high yields of the hydrolase.

REFERENCES:
patent: 4665029 (1989-05-01), Iwai et al.
Suigiura, M., "Bacterial Lipases", In: Lipases, Borgstrom and Brockman (eds) Elsevier; Amsterdam, New York, Oxford; 1984.
Macrae, A. R., "Extracellular Microbial Lipases", In: Microbial Enzymes and Biotechnology, Fogarty, W. (ed); Applied Science Publishers, N.Y. 1984.

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