Lipase determination method and reagent

Details Lipase determination method and reagent Lipase determination method and reagent

1979-07-13

1981-07-21

Therkorn, Ernest G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435184, 435810, 23230B, C12Q 144

Patent

active

042799945

ABSTRACT:
An improved reagent and method for determination of lipase in biological fluids utilizes an S-acyl compound as the substrate and a chromogenic sulfhydryl reagent to develop a measureable color. The improved reagent and method include a solid, water soluble pseudocholinesterase inhibitor, preferably eserine salicylate. Albumin may also included to increase sensitivity. Lipase is measured by incubating a specimen with the S-acyl compound, chromogenic sulfhydryl reagent and pseudocholinesterase inhibitor; terminating the incubation by adding a non-ionic surfactant; measuring the resulting color directly, and comparing the color to that obtained with a chemical standard solution containing an S-(2-aminoalkyl) isothiouronium salt.

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Chemical Abstracts, vol. 81, 21034b (1974).

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