Linear polyethylenimine-sterol conjugates for gene delivery

Details Linear polyethylenimine-sterol conjugates for gene delivery Linear polyethylenimine-sterol conjugates for gene delivery

2007-02-27

2007-02-27

Badio, Barbara P. (Department: 1617)

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Carbohydrate doai

C514S171000, C552S544000

Reexamination Certificate

active

10623020

ABSTRACT:
Linear polyethylenimine was modified with sterols, such as cholesterol, in three different geometries: linear shaped (L), T-shaped (T), and a combined linear- and T-shaped (LT), to result in linear polyethylenimine-sterol conjugates. These conjugates were mixed with nucleic acids to form complexes for delivery of the nucleic acids into cells. Mammalian cells transfected with these complexes showed protein expression levels higher than linear polyethylenimine alone, and twice that of branched polyethylenimine, but without any significant loss in cell viability. Methods of making these compositions and methods of using them for gene delivery are also described.

REFERENCES:
patent: 5783565 (1998-07-01), Lee et al.
patent: 5935936 (1999-08-01), Fasbender et al.
D.Y. Furgeson, R.N. Cohen, R.I. Mahato & S.W. Kim, Novel Water Insoluble Lipoparticulates for Gene Delivery, 19 Pharm. Res. 382-390 (2002).
Affleck, D.G., Yu, L., Bull, D.A., Bailey, S.H., & Kim, Augmentation of myocardial transfection using TerplexDNA: a novel gene delivery system, 8 Gene Ther. 349-353 (2001).
Lee, M., Rentz, J., Han, S.O., Bull, D.A., & Kim, S.W., Water-soluble lipopolymer as an efficient carrier for gene delivery to myocardium, 10 Gene Ther. 585-593 (2003).

Affiliated with

Also associated with

Rating

Linear polyethylenimine-sterol conjugates for gene delivery does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Linear polyethylenimine-sterol conjugates for gene delivery, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Linear polyethylenimine-sterol conjugates for gene delivery will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3881251