Limulus lysate having improved capacity to precipitate in the pr

Details Limulus lysate having improved capacity to precipitate in the pr Limulus lysate having improved capacity to precipitate in the pr

1974-07-17

1978-06-20

Padgett, Benjamin R.

Compositions

Nonreactive analytical, testing, or indicating compositions

23230B, 1951035R, 424 2, 424 12, 424 95, 424101, 424253, C09K 300, G01N 3316, G01N 3100, C12K 104

Patent

active

040960919

ABSTRACT:
A family of catalysts is disclosed for providing improved capacity for Limulus lysate solution to precipitate in the presence of extremely low concentrations of endotoxin. The catalyst family includes imidazole, manganese ions, Cleland's reagent, magnesium ions, strontium ions, barium ions, cysteine, lithium ions, and various combinations of the above materials. Solutions of the above catalysts may be used to advantage to reconstitute lyophilized Limulus lysate.

REFERENCES:
patent: 3954663 (1976-05-01), Yamamoto et al.
E. Thye Yin, et al., Biochim. Biophys. Acta, vol. 261, pp. 284-289 (1972).
Bryan, F. T., et al., Science, vol. 144, pp. 1147-1148 (29 May 1964).
Chemical Abstracts, vol. 78, No. 95202q (1973).
Chemical Abstracts, vol. 79, No. 89101k (1973).
Young, N. S. et al., J. Clinical Investigation, vol. 51, pp. 1790-1797 (1972).

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