Chemistry: analytical and immunological testing – Biospecific ligand binding assay
Patent
1982-07-12
1986-01-14
Kepplinger, Esther M.
Chemistry: analytical and immunological testing
Biospecific ligand binding assay
436 63, 436172, 436513, 436518, 436534, 436805, 356317, 356318, 356335, 356379, 356417, 2504591, 2504611, 2504612, 422 73, G01N 3354, G01N 2164, G01N 3350
Patent
active
045645981
ABSTRACT:
A method and apparatus for determining the presence of particles in a fluorescent dispersion which exceed either a predetermined fluorescence intensity or size are disclosed. The method and apparatus involve the irradiation of the dispersion with excitation light, the examination of the fluorescent light entering an optical fiber from a portion of the excited region small enough to permit only a low probability that more than one particle of interest is present in the volume during a predetermined period of time, and the measurement of the emitted fluorescence entering the fiber, substantially free of excitation light, to the extent that the fluorescence differs in intensity from that of the dispersion in the absence of the particle.
REFERENCES:
patent: 4199559 (1980-04-01), Ullman et al.
patent: 4421860 (1983-12-01), Elings et al.
O. Adawi and M. E. Weissman, Biophysical Journal (1981) 33:188a.
Journal of Immunological Methods, 47, 13-24 (1981) Miller, et al., "Usage of the Flow Cytometer-Cell Sorter".
Int. J. Immunopharmac., 3(3), 249-254 (1981) Hoffman, et al., "Immunofluorescent Analysis of Blood Cells by Flow Cytometry".
Science, 212, 1266-1267 (1981) Briggs, et al., "Homogeneous Fluorescent Immunoassay".
Proc. Natl. Acad. Sci. USA, 77(8), 4904-4908 (1980) Nicoli, et al., "Fluorescence Immunoassay Based on Long Time Correlations of Number Fluctuations".
Kepplinger Esther M.
Leitereg Theodore J.
Rowland Bertram I.
Syntex (U.S.A.) Inc.
White P. K.
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