Limited enzyme assay for aminotransferases

Details Limited enzyme assay for aminotransferases Limited enzyme assay for aminotransferases

1993-04-02

1994-11-22

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435 18, 435 24, 435808, 435810, 422 58, C12Q 152, C12Q 134, G01N 2100

Patent

active

053668707

ABSTRACT:
Method for determining the presence of an aminotransferase (AT.sub.1), other than aspartate aminotransferase (AST), in a biological sample. The method includes contacting the sample with an amino donor substrate for AT.sub.1, an amino acceptor substrate for AT.sub.1, cysteine sulfinic acid (CSA) in excess, a second aminotransferase (AT.sub.2), different from AT.sub.1, in excess, and an indicator for the presence of sulfite ions, under conditions in which the concentration of said AT.sub.1 can be determined by the amount of the indicator which forms a detectable signal.

REFERENCES:
patent: 3875014 (1975-04-01), Forgione
patent: 4241179 (1980-12-01), Madappally
patent: 4801535 (1989-01-01), Babler
patent: 4897347 (1990-01-01), Katsuyama
patent: 4923800 (1990-05-01), Ly
patent: 4981787 (1991-01-01), Baram
patent: 5047328 (1991-09-01), Chambers

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