Lignin-blocking treatment of biomass and uses thereof

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C530S378000, C435S068100, C435S099000

Reexamination Certificate

active

07604967

ABSTRACT:
Disclosed is a method for converting cellulose in a lignocellulosic biomass. The method provides for a lignin-blocking polypeptide and/or protein treatment of high lignin solids. The treatment enhances cellulase availability in cellulose conversion. Cellulase efficiencies are improved by the protein or polypeptide treatment. The treatment may be used in combination with steam explosion and acid prehydrolysis techniques. Hydrolysis yields from lignin containing biomass are enhanced 5-20%, and enzyme utilization is increased from 10% to 50%. Thus, a more efficient and economical method of processing lignin containing biomass materials utilizes a polypeptide/protein treatment step that effectively blocks lignin binding of cellulase.

REFERENCES:
patent: 4009075 (1977-02-01), Hoge
patent: 4237226 (1980-12-01), Grethlein
patent: 4556430 (1985-12-01), Converse et al.
patent: 4668340 (1987-05-01), Sherman
patent: 4708746 (1987-11-01), Hinger
patent: 4746401 (1988-05-01), Roberts et al.
patent: 4861721 (1989-08-01), Waterbury et al.
patent: 5125977 (1992-06-01), Grohmann et al.
patent: 5258293 (1993-11-01), Lynd et al.
patent: 5424417 (1995-06-01), Torget et al.
patent: 5503996 (1996-04-01), Torget et al.
patent: 5529663 (1996-06-01), Springer
patent: 5536325 (1996-07-01), Brink
patent: 5688674 (1997-11-01), Choi et al.
patent: 5705369 (1998-01-01), Torget et al.
patent: 5837506 (1998-11-01), Lynd et al.
patent: 6022419 (2000-02-01), Torget et al.
patent: 6063204 (2000-05-01), Hester et al.
patent: 6159510 (2000-12-01), Lizak
patent: 7018213 (2006-03-01), Marcus et al.
patent: 0 268 575 (1988-05-01), None
patent: 24738 (1914-10-01), None
patent: WO 94/29474 (1994-12-01), None
Minami et al. Characterization of clarified medium from submerse and semisolid cultivation of OF Aspergillus awamori NRRL3112 by size-exclusion chromatography. Braz. J. Chem. Eng. vol. 16 No. 2 Jun. 1999. pp. 1-7.
Wyman, Charles E., “Biomass Ethanol: Technical Progress, Opportunities, and Commercial Challenges”, Annu. Rev. Energy Environ. 1999, V. 24, pp. 189-226.
Wyman, Charles E., Spindler, Diane D., and Grohmann, Karel, “Simultaneous Saccharification and Fermentation of Several Lignocellulosic Feedstocks to Fuel Ethanol”, Biomass and Bioenergy, 1992, vol. 3, No. 5, pp. 301-307, Pergamon Press Ltd., Great Britian.
Wyman, Charles E., “Ethanol from Lignocellulosic Biomass: Technology, Economics, and Opportunities”, Bioresource Technology 50, 1994, pp. 3-16, Elsevier Science Limited, Great Britain.
Wyman, C.E., Spindler, D.D., Grohmann, K. and Lastick S.M., “Simultaneous Saccharification and Fermentation of Cellulose with the YeastBrettanomyces clausenii”, Biotechnology and Bioengineering Symp. No. 17, 1986, pp. 221-238.
Wyman, Charles E., “Twenty Years of Trials, Tribulations, and Research Progress in Bioethanol Technology”, Applied Biochemistry and Biotechnology, 2001, V. 91-93, pp. 5-21, The Humana Press Inc.
Wright, John D., Wyman, Charles E. and Grohmann, Karel, “Simultaneous Saccharification and Fermentation of Lignocellulose”, 1988, pp. 75-90, The Humana Press Inc.
Spindler, Diane D., Wyman, Charles E., Mohagheghi, Ali, and Grohmann, Karel, “Thermotolerant Yeast for Simultaneous Saccharification and Fermentation of Cellulose to Ethanol”, 1988, pp. 279-293, The Humana Press Inc.
Spindler, Diane D., Wyman, Charles E., Grohmann, Karel and Philippidis, George P., “Evaluation of the Cellobiose-Fermenting YeastBrettanomyces custersiiin the Simultaneous Saccharification and Fermentation of Cellulose”, Biotechnology Letters, May 1992, V. 14 No. 5, pp. 403-407.
Spindler, Diane D., Wyman, Charles E., and Grohmann, Karel, “The Simultaneous Saccharification and Fermentation of Pretreated Woody Crops to Ethanol”, Applied Biochemistry and Biotechnology, 1991, vol. 28/29, pp. 773-786.
Spindler, Diane D., Wyman, Charles E., Grohmann, Karel and Mohagheghi, Ali, “Simultaneous Saccharification and Fermentation of Pretreated Wheat Straw to Ethanol with Selected Yeast Strains and B-Glucosidase Supplementation”, Applied Biochemistry and Biotechnology, 1989, vol. 20/21, pp. 529-540.
Spindler, Diane, Wyman, Charles and Grohmann, Karel; “Evaluation of Pretreated Herbaceous Crops for the Simultaneous Scaccharification and Fermentation Process”, Applied Biochemistry and Biotechnology, 1990, vol. 24/25, pp. 275-286.
Yang, Bin, Boussaid, Abdel, Mansfield, Shawn D., Gregg, David J. and Saddler, John N., “Fast and Efficient Alkaline Peroxide Treatment to Enhance the Enzymatic Digestibility of Steam-Exploded Softwood Substrates”, Biotechnology and bioengineering, Mar. 20, 2002, vol. 77, No. 6, pp. 678-684.
Sewalt, V.J.H., Glasser, W.G. and Beauchemin, K.A., “Lignin Impact on Fiber Degradation. 3. Reversal of Inhibition of Enzymatic Hydrolysis by Chemical Modification of Lignin and by Additives”, J. Agric. Food Chem, 1997, vol. 45, No. 5, pp. 1823-1828, American Chemical Society.
Mohagheghi, A., Tucker, M., Grohmann, K. and Wyman, C., “High Solids Simultaneous Saccharification and Fermentation of Pretreated Wheat Straw to Ethanol”, Applied Biochemistry and Biotechnology, 1992, vol. 33, pp. 67-81, The Humana Press Inc.
Lu, Yanpin, Yang, Bin, Gregg, David, Saddler, John N. and Mansfield, Shawn D., “Cellulase Adsorption and an Evaluation of Enzyme Recycle During Hydrolysis of Steam-Exploded Softwood Residues”, Applied Biochemistry and Biotechnology, 2002, vol. 98-100, pp. 641-654, Humana Press Inc.
Eriksson, Tony, Borjesson, Johan and Tjerneld, Folke, “Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose”, Enzyme and Microbial Technology 2002, v. 31, pp. 353-364, Elsevier Science Inc.
Torget, R., Himmel, M. and Grohmann, K.; “Dilute-Acid Pretreatment of Two Short-Rotation Herbaceous Crops”, Applied Biochemistry and Biotechnology, 1992, vol. 34/35, pp. 115-123, The Humana Press Inc.
Sutcliffe, Roger and Saddler, John N., “The role of Lignin in the Adsorption of Cellulases during Enzymatic Treatment of Lignocellulosic Material”, Biotechnology and Bioengineering Symp. No. 17, 1986, pp. 749-762.
Wu, Michael M., Chang, Kevin, Gregg, David J., Boussaid, Abdel, Beaston, Rodger P. and Saddler, John N. “Optimization of Steam Explosion to Enhance Hemicellulose Recovery and Enzymatic Hydrolysis of Cellulose in Softwoods”, Applied Biochemistry and Biotechnology, 1999, vol. 77-79, pp. 47-54, The Humana Press Inc.
Grohmann, K., Torget, R. and Himmel, M., “Dilute Acid Pretreatment of Biomass at High Solids Concentrations”, Biotechnology and Bioengineering Symp. No. 17, 1986, pp. 135-151.
Chernoglazov, Vladimir M., Ermolova, Olda V. and Klyosov, Anatole A., “Adsorption of high-purify endo-1,4-β-glucanases fromTrichoderma reeseion components of lignocellulosic materials: cellulose, lignin, and xylan”, Enzyme Microb. Technol., 1988, vol. 10, August, pp. 503-507.
Boussaid, Abdel, Robinson, Jamie, Cai, Yi-Jin, Gregg, David J. and Saddler, John N., “Fermentability of the Hemicellulose Derived Sugars from Steam-Exploded Softwood (Douglas Fir)”, Int'l Conf. Biotechnol. Pulp Pap. Ind., 7th, 1998, pp. C239-C242.
Gould, Michael J., “Alkaline Peroxide Delignification of Agricultural Residues to Enhance Enzymatic Saccharification”, Biotechnology and Bioengineering, 1984, vol. XXVI, pp. 046-052, John Wiley & Sons, Inc.
Ghose, T.K., Roychoudhury, P.K. and Ghosh, P., “Simultaneous Saccharification and Fermentation (SSF) of Lignocellulosics to Ethanol Under Vacuum Cycling and Step Feeding”, Biotechnology and Bioengineering, 1984, vol. XXVI, pp. 377-381, John Wiley & Sons, Inc.
Overend, R.P. and Chornet, E., “Fractionation of Lignocellulosics by Steam-Aqueous Pretreatments”, Philosophical Transactions of the Royal Society of London, Series A, Mathematical and Physical Sciences, Apr. 30, 1987, vol

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Lignin-blocking treatment of biomass and uses thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Lignin-blocking treatment of biomass and uses thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Lignin-blocking treatment of biomass and uses thereof will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-4055538

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.