Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-05-02
1998-12-01
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 5, 536 243, 536 2432, 536 2433, 422 51, 385130, C12Q 168, C12Q 170, C07H 2104, G01N 2520
Patent
active
058436510
ABSTRACT:
A waveguide binding assay method involves detecting the scattering of light directed into the waveguide, the scattering being the result of scattering labels specifically bound to the waveguide within the penetration depth of an evanescent wave. The waveguide may be transparent plastic or glass and the binding is typically by oligonucleotide hybridization or immunological capture. Light scattering labels include colloidal metals or non-metals, including gold, selenium and latex. A light absorbing member consisting of dye or concentrated particles may also be employed to enhance signal. Real-time binding and dissociation can be monitored visually or by video imaging, such as with a CCD camera and frame grabber software. Hybridization mismatches of as few as one base can be distinguished by real-time melting curves.
REFERENCES:
patent: 5192510 (1993-03-01), Zoha et al.
Gordon Julian
Hoijer Joanell V.
Stimpson Donald Irvine
Abbott Laboratories
Brainard Thomas D.
Jones W. Gary
Rees Dianne
Yasger Paul D.
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