Light scatter-based immunoassay without particle self aggregatio

Details Light scatter-based immunoassay without particle self aggregatio Light scatter-based immunoassay without particle self aggregatio

1994-08-05

1996-12-31

Housel, James C.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is inorganic

436533, 436541, 436164, 435 71, G01N 33538, G01N 33544, G01N 33553

Patent

active

055894011

ABSTRACT:
A homogeneous immunoassay method for the simultaneous determination of one or more antibody, antigen or hapten analytes in a fluid sample, that comprises the quantification of the effect of said analytes on the statistical changes in a dimension of a light scatter pulse height distribution histogram of relatively large diameter monodisperse binding molecule-coated polymeric microspheres induced by the binding to said microspheres of polydisperse binding molecule-coated colloid metal particles of relatively small diameter. For simultaneous assays of multiple analytes, different diameter or refractive index microspheres are assigned to each analyte. The assay may be used in forward binding, displacement, inhibition, and competition type systems, with the direction of the change in histogram dimension depending on the system. A convenient dimension to measure is the normalized peak width of a graphical representation of the histogram. For simultaneous assays for multiple analytes, a monodisperse polymeric microsphere of unique diameter or refractive index is dedicated to each analyte, so as to generate multiple histograms, one for each analyte. Polydisperse binding molecule-coated colloid metal particles, when used in effective concentrations, will also serve as a scavenger means to reduce the interfering effects of non-specific substances in the analyte-containing fluid samples, particularly when said samples are of biological origin.

REFERENCES:
patent: 3873204 (1975-03-01), Friedman et al.
patent: 4305925 (1981-12-01), Kapmeyer et al.
patent: 4313734 (1982-02-01), Leuvering
patent: 4446239 (1984-05-01), Tsuji et al.
patent: 4480042 (1984-10-01), Craig et al.
patent: 4581334 (1986-04-01), Kirchanski
patent: 4853335 (1989-08-01), Olsen et al.
patent: 5017009 (1991-05-01), Schutt et al.
patent: 5079172 (1992-01-01), Hari et al.
patent: 5162863 (1992-11-01), Ito
patent: 5286452 (1994-02-01), Hansen
H. Bauer, et al. Experientia, 31:1149-1151 (1975).
M. Horisberger, et al. J. of Histochem. and Cytochem., 25(No. 4): 295-305 (1977).
M. Horisberger. Biol. Cellularie, 36:253-258 (1979).
Sakai et al., Chem. Pharm. Bull. 37(11):3010-14 (1989).
J. H. W. Leuvering et al., J. of Immunological Methods, 62:163-74 (1983).
J. H. W. Leuvering et al., J. of Immunological Methods, 45:183-94 (1981).
F. Wielaard et al., J. of Virological Methods, 17:149-158 (1987).
Duke Scientific Corporation Bulletin 88C Sep. 1, 1990 "Covaspheres Reagants".
Duke Scientific Corporation Bulletin 93D Apr. 1, 1992 "Flourescent Microspheres & Particles".
Horisberger et al., Experientia, 31:1147-9 (1975).
Rohr et al., Analytical Biochemistry, 182, 388-398 (1989).
Saunders, G. C. et al., Clinical Chemistry 31(12):2020-2023 (1985).
Van Erp et al., J. Immunoassay, 12:425-443 (1991).
Odell et al., Principles of Competitive Protein-Binding Assays, ed. 13:243-254 (1983).

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