Light-regulated promoters for production of heterologous protein

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4352544, 536 241, C12N 115, C12N 1509, C12N 1511, C07H 2104

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057416656

ABSTRACT:
The al-3 and related promoters can be used to provide light-regulated recombinant production of heterologous proteins in filamentous fungi. Expression systems utilizing these promoters can be placed in vectors which also optionally contain selectable marker means.

REFERENCES:
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Nelson et al., "Molecular Cloning of a Neurospora crassa Carotenoid Biosynthetic Gene (Albino-3) Regulated by Blue Light and the Products of the White Collar Genes", Mol. Cell. Biol., 9:1271-76 (1989).
Schmidhauser et al., "Cloning, Sequence, and Photoregulation of al-1, a Carotenoid Biosynthetic Gene of Neurospora crassa", Mol. Cell. Biol., 10:5064-70 (1990).
Baima, "Photoinduction of albino-3 Gene Expression in Neurospora crassa Conidia", J. Photochem. Photobiol., 15:233-38 (1992).

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