Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-10-30
1999-03-09
Spiegel, Carol A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 71, 435 25, 435968, 422 8207, 422 8208, 436800, 546102, 546104, 546107, C12Q 168
Patent
active
058798889
ABSTRACT:
A light emitting method of an acridinium ester, comprising reacting said acridinium ester and a superoxide anion, and a method of detecting a substance to be examined, comprising detecting a light emitted by reacting a superoxide anion with an acridinium ester used as a label are described. It is possible to carry out the reaction not under strongly alkaline conditions but around the neutral point and to generate strong luminescence which is stable over a long period of time.
REFERENCES:
patent: 4478817 (1984-10-01), Campbell et al.
patent: 4927769 (1990-05-01), Chang et al.
patent: 4933276 (1990-06-01), Baret
patent: 4946958 (1990-08-01), Campbell et al.
patent: 5306624 (1994-04-01), Roelant
H. Ischiropoulos et al, "Effect of Interferon Inducers on Superoxide Anion Generation from Rat Liver Microsomes Detected by Lucigenin Chemiluminescence," Biochem.Biophys. Res. Comm. 161(3):1042-48 (1989).
Kricka, "Chemiluminescent and Bioluminescent Techniques," Clin. Chem. 37(9):1472-81 (1991).
J. Littig, "Quantitation of Acridinium Esters Using Electrogenerated Chemiluminescence and Flow Injection," Anal. Chem. 64(10):1140-44 (15 May 1992).
T. Peters et al, "Lucigenin Chemiluminescence as a Probe for Measuring Reactive Oxygen Species Production in E. coli," Anal. Biochem 186:316-19 (1990).
M. Tosi et al, "A Rapid, Specific Assay for Superoxide Release from Phagocytes in Small Volumes of Whole Blood," Am. J. Clin. Path., 97(4):566-575 (Apr. 1992).
A. White et al, Principles of Biochemistry, 4.sup.th edition (McGraw-Hill Book Company New York 1968) p. 220.
Aizawa Masuo
Ikariyama Yoshihito
Kobatake Eiry
Ogasawara Minoru
Tanaka Mitsunao
Iatron Laboratories Inc.
Spiegel Carol A.
LandOfFree
Light emitting method of acridinium derivative and method of det does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Light emitting method of acridinium derivative and method of det, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Light emitting method of acridinium derivative and method of det will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1319628