Light controlled gene expression utilizing heterologous...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C536S023400

Reexamination Certificate

active

06887688

ABSTRACT:
This invention relates to the field of gene expression. In particular this invention relates to the use of heterologous phytochromes to translocate polypeptides into the nucleus of a cell. Where the polypeptides comprise transactivators or repressors this invention provides a system for light-directed gene expression.

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Yamaguchi et al. Light-dependent Translocation of a Phytochrome B-GFP Fusion Protein to the Nucleus in Transgenic Arabidopsis. The Journal of Cell Biology vol. 145 No. 3 1999.*
Taiz et al. Phytochrome Chapter 17, Plant Physiology 2ndEd. Sinauer Associates, Inc. Publishers, p. 487 1998.*
Kircher et al. Light Quality-Dependent Nuclear Import of the Plant Photoreceptors Phytochrome A and B The Plant Cell vol. 11 pp. 1999 1145-1156.*
Dorland's Illustrated Medical Dictionary 1988, p. 113, 1362.*
Kosugi et al. Interactions of the Arabidopsis E2F and DP Proteins Confers Their Concommitant Nuclear Translocation and Transactivation Plant Physiology 2002 vol. 128 pp. 833-843.*
Guralnick et al. The Plant Cell Transport of DNA into the Nuclei of Xenopus Oocytes by a Modified VirE2 Protein of Agrobacterium 1996 vol. 8 363-373.*
Hisada et al. (2000) “Light-induced Nuclear translocation of Endogenous Pea Phytochrome A Visualized by Immunocytochemical Procedures”, The Plant Cell, vol. 12, 1063-1078.
Lin et al. Sequence Listing Search Result.

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