Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-07-14
1997-05-27
LeGuyader, John L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 9131, 4351721, 4351723, 536 231, 536 232, C12Q 168, C12N 1511, C07H 2104
Patent
active
056331339
ABSTRACT:
The claimed invention is drawn to a method for ligation of an enzymatic nucleic acid molecule having a hammerhead motif to a separate nucleic acid molecule. The method includes use of ligation enhancing conditions in which ligation is enhanced at least two fold over that observed in a medium containing only Mg.sup.2+ as a divalent cation at 20.degree. C.
REFERENCES:
Haseloff et al. Nature 334:585 (1988).
Bartel and Szostak, "Isolation of New Ribozymes From a Large Pool of Random Sequences," Science 261:1411-1418 (1993).
Berzal-Herranz et al., "In vitro selection of active hairpin ribozymes by sequential RNA-catalyzed clevage and ligation reactions," Genes & Development 6:129-134 (1992).
Berzal-Herranz et al., EBMO J. 12:2567-2574 (1993).
Dahm and Uhlenbeck, Biochemistry 30:9464-9469 (1991).
Hertel et al., "A Kinetic Thermodynamic Framework for the Hammerhead Ribozyme Reaction," Biochemistry 33:3374-3385 (1994).
Izatt et al., "Sites and Thermodynamic Quantities Associated with Proton and Metal Ion Interaction with Ribonucleic Acid, Deoxyribonucleic Acid, and Their Constituent Bases, Nucleosides and Nucleotides," Chem. Rev. 71:439-481 (1971).
Joyce, "Directed Molecular Evolution," Scientific American 267:90-97 (1992).
Koizumi and Ohtsuka, Biochemistry 30:5145-5150 (1991).
Perreault et al., "Mixed Deoxyribo-and Ribo-Oligonucleotides with Catalytic Activity," Nature 344:565-567 (1990).
Pieken et al., "Kinetic Characterization of Ribonuclease-Resistant 2'-Modified Hammerhead Ribozymes," Science 253:314-317 (1991).
Rossi et al, "Ribozymes as Anti-HIV-1 Therapeutic Agents: Principles, Applications, and Problems," Aids Research and Human Retroviruses 8:183 (1992).
Usman and Cedergren, "Exploiting the chemical synthesis of RNA," TIBS 17:334-339 (1992).
Hertel Klemens J.
Long David M.
Uhlenbeck Olke C.
LandOfFree
Ligation with hammerhead ribozymes does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Ligation with hammerhead ribozymes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Ligation with hammerhead ribozymes will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2328314