Ligation of double-stranded DNAs

Details Ligation of double-stranded DNAs Ligation of double-stranded DNAs

2006-04-04

2006-04-04

McKelvey, Terry (Department: 1633)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S069100, C435S091420, C435S252300, C435S183000

Reexamination Certificate

active

07022501

ABSTRACT:
The present invention relates to a DNA ligation method. Specifically, a DNA complex comprising a three-stranded structure in each of the ends of double-stranded DNAs, one of which has single-stranded regions at the end, and the other of which does not have single-stranded regions at the end, is formed under the presence of a homologous recombinant protein. Moreover, according to needs, the DNA complex is introduced into host cells, and said cells are cultured.

REFERENCES:
patent: 5460941 (1995-10-01), Camerini-Otero et al.
patent: 6541226 (2003-04-01), Shigemori et al.
patent: WO97/04111 (1997-02-01), None
patent: WO-97/04111 (1997-02-01), None
R. Daniel Camerini-Otero et al. Homologous Recombination Proteins in Prokaryotes and Eukaryotes Annu. Rev. Genetics 1995 29:509-52.
John G. K. Williams et al.Escherichia coliRecA Protein Protects Single-stranded DNA of Gapped Duplex DNA from Degradation by RecBC DNase* The Journal of Biological Chemistry vol. 256 No. 14 Jul. 25, pp. 7573-7582 1981.
Fujiwara et al., Direct cloning by covalent attachment of probe DNA to target DNA, 1998, Nucleic Acids Research, vol. 26, pp. 5734-5737.
Fujiwara et al., Direct probing: covalent attachment of porbe DNA to double-stranded target DNA, Nucleic Acids Research, vol. 26, pp. 5728-5733.
Cimmino et al., Ligation of nonmatching DNA molecule ends, 1995, PLASMID, vol. 34, pp. 1-10.
Fujiwara et al.,Nucleic Acids Research, 26(24):5734-5737 (1998).
Fujiwara et al.,Nucleic Acids Research, 26(24):5728-5733 (1998).
Aslanidis et al.,Nucleic Acids Research, 18(20):6069-6074 (1990).
Martin Teintze,RecA-Assisted Rapid Enrichment of Specific Clones from Model Dna Libraries, Biochemical and Biophysical Research Communications, 211(3): 804-811 (1995), Academic Press, Inc.

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