Ligation assay

Details Ligation assay Ligation assay

2007-08-14

2007-08-14

Whisenant, Ethan (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091200, C536S023100, C536S024300

Reexamination Certificate

active

10866012

ABSTRACT:
Disclosed are methods and kits for detecting or quantifying one or more target polynucleotide sequences in a sample. Embodiments of the invention employ a first ligation reaction, a subsequent optional amplification reaction, and a second ligation reaction. Embodiments of the invention combine the specificity of both hybridization and ligation reactions along with universal probe polynucleotide sequences to achieve specific and multiplexed detection of a plurality of target polynucleotide sequences.

REFERENCES:
patent: 5674679 (1997-10-01), Fuller
patent: 5854033 (1998-12-01), Lizardi
patent: 2002/0150921 (2002-10-01), Barany et al.
patent: 2004/0029142 (2004-02-01), Schon
patent: 2004/0058330 (2004-03-01), Aevarsson et al.
patent: 2004/0110134 (2004-06-01), Wenz et al.
patent: 2006/0141475 (2006-06-01), Rosenblum et al.
patent: 1 130 113 (2000-02-01), None
patent: WO97/04128 (1997-02-01), None
patent: WO 02/053778 (2002-07-01), None
patent: WO 03/002762 (2003-01-01), None
Zhang et al., Whole genome amplification from a single cell: Implications for genetic analysis. PNAS 89 : 5847-5851 (1992).
International Preliminary Report and Written Opinion dated Dec. 13, 2005, issued in International Application No. PCT/US2004/018396, 7 pages.

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